[Histonet] RE: fish eggs: processing and fixation

[Debbie Faichney]

Hi Christy,

Do you have the facility for resin sectioning?  This is the way to go for fish eggs.  It's a long time since this type of processing was carried out in our labs but if you are able to process in this way I'm sure I would be able to dig out a protocol from our Thesis library.  I also suspect 10%NBF will have to be replaced with another fixative.

Regards

Debbie
Technical Specialist
Fish Health and Welfare Laboratories
Institute of Aquaculture
University of Stirling
Stirling, FK9 4LA
Tel: +44(1)1786 466592/466590





-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Thompson, Christy
Sent: 23 October 2014 19:39
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] fish eggs: processing and fixation

Hi all, I'm currently working on some unfertilized fish eggs. They were fixed in 10% NBF and processed in a vacuum processor with paraffin. The eggs are extremely hard to section and most of the yolk goes AWOL on the way to the water bath.  I did do a search here for some help and a few things were posted but one post in particular found some answers but they were not listed in his reply and I think the post was pretty old (but no date on it).  Anyone out there have any success with sectioning fish eggs?
Thanks in advance!

Christy Thompson
A/Histology Lab Leader - Aquatic Animal Health Pacific Biological Station Fisheries and Oceans Canada Nanaimo, BC
250-756-7060
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