[Histonet] Fixation in concentrated 37% formaldehyde

[Rene J Buesa]

Using "pure" formaldehyde as a fixative is not a good idea (you already know that) and the staining will be affected BUT, on the other hand, IHC will only require stronger HIER (more time and temperature) but other than that I do think the epitopes will be detected. So the "effects" will be mostly "cosmetic".René J.   

     On Wednesday, October 22, 2014 11:04 AM, Orla M Gallagher <o.m.gallagher <@t> sheffield.ac.uk> wrote:
   

 Dear Histonetters,

What would the effect be of fixing tissues samples in concentrated
formaldehyde instead of 10% buffered formalin? One of our researchers would
like us to prepare some bones for histology staining which have been fixed
in 37% formaldehyde by mistake and stored for 4 years. I assume there will
be formalin pigment in these samples, so even a H&E may not look great,
while any enzyme histochemistry or immunohistochemistry is probably not
worth trying. I haven't seen the samples yet as they will be arriving from
another UK university.

Has anyone out there fixed any tissues in concentrated formaldehyde by
accident or by design?

Thanks,
Orla

-- 
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
UK

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:        0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


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