[Histonet] Fixation in concentrated 37% formaldehyde

[Orla M Gallagher]

Dear Histonetters,

What would the effect be of fixing tissues samples in concentrated
formaldehyde instead of 10% buffered formalin? One of our researchers would
like us to prepare some bones for histology staining which have been fixed
in 37% formaldehyde by mistake and stored for 4 years. I assume there will
be formalin pigment in these samples, so even a H&E may not look great,
while any enzyme histochemistry or immunohistochemistry is probably not
worth trying. I haven't seen the samples yet as they will be arriving from
another UK university.

Has anyone out there fixed any tissues in concentrated formaldehyde by
accident or by design?

Thanks,
Orla

-- 
**************************
Ms. Orla Gallagher
Bone Analysis Laboratory
Mellanby Centre for Bone Research
Department of Human Metabolism
D Floor Medical School
University of Sheffield
Beech Hill Road
Sheffield
S10 2RX
UK

Website: http://mellanbycentre.dept.shef.ac.uk

Tel:         0044114-2713337 (office)
              0044114-2713174 (lab)
E-Mail:    o.m.gallagher <@t> sheffield.ac.uk


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