[Histonet] RE: Gram staining

[Gomez, Milton]

we use 5ml Picric Acid to 50 ml acetone and it works very well.  

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Cooper, Brian [bcooper <@t> chla.usc.edu]
Sent: Thursday, October 09, 2014 3:48 PM
To: Bitting, Angela K.; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Gram staining

We use the McDonald's Gram Stain kit from American Master Tech, which employs the use of a Modified Tartrazine counterstain.  Both our docs and techs love our Gram stains . . .

Here's the link to the product info.

http://www.americanmastertech.com/store/main.aspx?p=ItemDetailStyles&item=KTMGS

Thanks,

Brian D. Cooper, HT (ASCP)CM | Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357     Pager: 213-209-0184
bcooper <@t> chla.usc.edu


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bitting, Angela K.
Sent: Thursday, October 09, 2014 11:36 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Gram staining


Our new Pathologist would like to see more yellow in the background of our Gram stain.  We use picric acid-acetone currently, which looks more pink than yellow most times. So  I'm wondering if a method using a Tartrazine counterstain would be better.
Any thoughts?


IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you.

Geisinger Health System utilizes an encryption process to safeguard Protected Health Information and other confidential data contained in external e-mail messages. If email is encrypted, the recipient will receive an e-mail instructing them to sign on to the Geisinger Health System Secure E-mail Message Center to retrieve the encrypted e-mail._______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



---------------------------------------------------------------------
CONFIDENTIALITY NOTICE: This e-mail message, including any attachments,
is for the sole use of the intended recipient(s) and may contain confidential
or legally privileged information. Any unauthorized review, use, disclosure
or distribution is prohibited. If you are not the intended recipient, please
contact the sender by reply e-mail and destroy all copies of this original message.

---------------------------------------------------------------------


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet