[Histonet] RE: Ebola

[Patsy Ruegg]

All samples should be handled with universal precautions assuming they may be infectious, formalin fixation is considered an eliminator of biologic hazard for most things like Ebola, but then there is always CJD that it does not work for.  I would not be doing any frozen sections on samples with suspected biologic hazard since they do not get fixed in formalin typically, although you can fix in formalin then rinse it out and infiltrate with sucrose to make fixed frozen sections.  Keep calm and use your common sense and vast experiences.

Cheers,
Patsy 

Here is a story for you.  Back in the late 70's early 80's when we didn't know much about HIV or HPV I worked in a Heme/Path lab at the U.  One of the pathologist I worked with was studying genital wort's and lesions getting her samples from the local sexually transmitted disease clinic.  This was back in the day when T&B cells had to be done on cell suspensions, frozen sections or smears/touch preps not aldehyde fixed.  The antibodies and detection systems did not yet work in FFPE tissues.  I prepared unfixed frozen samples on a ton of these biopsies.  I double gloved, used UP and got thru this.  Looking back on that project I do not think I would have done it knowing what I know now.   There is a reason or two that Histotechnology is the most dangerous job I guess.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net


From: JWatson <@t> gnf.org
To: Joyce.Weems <@t> emoryhealthcare.org; TanyaAbbott <@t> catholichealth.net; histonet <@t> lists.utsouthwestern.edu
Date: Thu, 9 Oct 2014 19:52:12 +0000
CC: 
Subject: [Histonet] RE: Ebola

Have it delivered in formalin and let it fix completely.
 
 
 
"Formalin fixation eliminates the biologic hazard associated with transporting samples containing infectious EBO virus, .................... Thus, formalin fixation provides an adequate noninfectious specimen for EHF diagnosis, ........................."
 
A quote from:
 
Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever 
 
Ethleen S. Lloyd, and  C. J. Peters, et al
 
 
+ Author Affiliations
 
Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization and US Agency for International Development and Ministry of Health, Kinshasa, Hôpital Général de Référence de Kikwit, Kikwit, and Mosango Mission Hospital, Mosango, Democratic Republic of the Congo; Médecins sans Frontières—Belgium, Brussels, Belgium 
 
 
+ Author Notes
 
↵* Current affiliation: Institut de Médecine Tropicale, Antwerp, Belgium (M.A.B.); Médecins sans Frontières—Belgium, N'Djamena, Chad (E.V.); World Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). 
 
Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333 (pyr3 <@t> cdc.gov).
 
 
James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel    858-332-4647
Fax   858-812-1915
jwatson <@t> gnf.org
 
 
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, October 09, 2014 12:22 PM
To: 'Abbott, Tanya'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola
 
Handle with universal precautions as always is our emphasis.
 
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
 
 
 
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
 
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Thursday, October 09, 2014 3:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ebola
 
Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection?
 
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabbott <@t> catholichealth.net
 
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