[Histonet] RE: Ebola

[Connolly, Brett M]

I did my postdoc research at USAMRIID on Ebola virus pathogenesis. Our standard fixation protocol for Ebola, Lassa and other BSL4 pathogens was to let them fix in formalin for a minimum of 30 days. The containers of fixed tissue were not allowed out of the P4 containment lab until the 30 day limit was up. Even then we had disinfectant dunk tanks to pass the sealed containers through to get them out of the P4  lab.  You can't be too careful with that bugger...just sayin'

Brett

Brett M. Connolly, Ph.D.
Principle Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of James Watson
Sent: Thursday, October 09, 2014 3:52 PM
To: 'Weems, Joyce K.'; 'Abbott, Tanya'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola

Have it delivered in formalin and let it fix completely.



"Formalin fixation eliminates the biologic hazard associated with transporting samples containing infectious EBO virus, .................... Thus, formalin fixation provides an adequate noninfectious specimen for EHF diagnosis, ........................."

A quote from:

Long-Term Disease Surveillance in Bandundu Region, Democratic Republic of the Congo: A Model for Early Detection and Prevention of Ebola Hemorrhagic Fever 

Ethleen S. Lloyd, and  C. J. Peters, et al
 

+ Author Affiliations

Centers for Disease Control and Prevention, Atlanta, Georgia; World Health Organization and US Agency for International Development and Ministry of Health, Kinshasa, Hôpital Général de Référence de Kikwit, Kikwit, and Mosango Mission Hospital, Mosango, Democratic Republic of the Congo; Médecins sans Frontières—Belgium, Brussels, Belgium 
 

+ Author Notes

↵* Current affiliation: Institut de Médecine Tropicale, Antwerp, Belgium (M.A.B.); Médecins sans Frontières—Belgium, N'Djamena, Chad (E.V.); World Health Organization, Kinshasa, Democratic Republic of the Congo (J.K.). 

Reprints or correspondence: Dr. Pierre E. Rollin, Special Pathogens Branch, NCID/DVRD, Mailstop G-14, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333 (pyr3 <@t> cdc.gov).


James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Scientific Technical Leader II, Histology
Tel    858-332-4647
Fax   858-812-1915
jwatson <@t> gnf.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Thursday, October 09, 2014 12:22 PM
To: 'Abbott, Tanya'; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Ebola

Handle with universal precautions as always is our emphasis.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
Sent: Thursday, October 09, 2014 3:04 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Ebola

Dare I ask?! Are any Pathology labs discussing what to do with specimens/precautions, etc. regarding a person with a potential Ebola infection?

Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology St. Joseph Medical Center Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
email: tanyaabbott <@t> catholichealth.net

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