[Histonet] embedding of insects in historesin (Technovit 7100)
Grantham, Andrea L - (algranth)
algranth <@t> email.arizona.edu
Fri Nov 21 09:41:54 CST 2014
First of all, this sounds like a Damien Laudier question. Damien, I hope you can help this guy.
Secondly, do you have to use plastic embedding? I was successful with paraffin embedding. I was able to section various small insects and also bees. It takes some patience and soaking in mollifex or glycerin water but the chitin became less brittle and able to section without ripping out the internal organs.
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Dr.Harst <@t> gmx.net [Dr.Harst <@t> gmx.net]
Sent: Friday, November 21, 2014 4:09 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] embedding of insects in historesin (Technovit 7100)
are there any experiences in embedding of insects in historesin (GMA
or Glycolmethacrylat (Technovit 7100))? I have tried Technovit after
fixing in Formaldehyd with poor results. The specimen tends to fail
out of the block. There is no connection between the cuticula and the
resin. Therefore it is not possible, to stretch the ribbon on the
surface of water. And I believe there is some interaction between the
chitin and the resin which makes trouble. The chitin is brittle after
embedding in resin. I have tried different times in the embedding
process, but without good results. Embedding single organs for example
ovaries is without any difficulties . Obviously chitin makes the
Perhaps helps another fixing or some other pretreatment?
Thanks for a little help !
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