[Histonet] Bone Marrow processing using Immunocal

Patsy Ruegg pruegghm <@t> hotmail.com
Mon Nov 17 14:54:00 CST 2014

I use a platform shaker and slosh the sample around while it is in decal solution at RT, just make sure it is well fixed before you decal, Immunocal is 5% formic acid decal solution it is not a fixative and it is a bit slower than some of the rapid decal reagents out there but is much better for IHC.  When I was in HemePath we would fix for about 6 hrs before decaling on the shaker for 2-4hrs depending on the size (thickness) of the bone bx.

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net

> Date: Mon, 17 Nov 2014 14:05:28 -0500
> From: Carolyn.Barnes2 <@t> va.gov
> To: Histonet <@t> lists.utsouthwestern.edu
> CC: 
> Subject: [Histonet] Bone Marrow processing using Immunocal
> I am looking for a Bone Marrow procedure that uses Immunocal as the
> decalcifier. I am particularly interested in how long they fix in
> Immunocal and if heat is used in any way . Our lab  just started using
> this product because we are under the impression that it gave better ISH
> results.
> Thank-you all so much for any assistance. I really appreciate everyone's
> help.
> Carolyn K. Barnes
> Histology Supervisor
> McGuire VA Medical Center
> Richmond, VA 23249
> Ph: 804-675-5000, x2158
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