[Histonet] TC nuclear stain

Patsy Ruegg pruegghm <@t> hotmail.com
Wed Nov 12 11:35:15 CST 2014

TC experts.  One of my former students is doing a TC stain and the nuclear stain with iron hematoxylin is washing out.  I remember that I had this problem myself with weigerts/iron heme, I always got a weaker purple stain rather than black like it was supposed to be.  Do you think it would help to repeat the nuclear stain at the end of the stain as well as in the beginning, make fresh hematoxylin, etc????

Thank you for your advise,


Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com
pruegg <@t> ihctech.net

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