[Histonet] anti-roll plate damage?

Bain,Virginia VEBain <@t> mdanderson.org
Fri Nov 7 16:01:24 CST 2014

Hello again,

First I wanted to thank everyone for their helpful replies.  To answer an
earlier question, yes we do use the Sakura low profile blades but we are
using them without issue on our microtome (also a low profile blade

I especially wanted to thank the folks at Thermo Scientific who upon
finding out that we were having trouble have been incredibly helpful.
They have been very nice about letting me know that I should never feel
that I cannot contact them.  Finally, in case anyone encounters an issue
like this in the future, their diagnosis was that some user may have been
cutting with the anti-roll plate too far forward.  Thanks again to
everyone for their assistance.


Virginia Bain
Postdoctoral Fellow
Richie Lab
M D Anderson - Science Park

On 11/3/14, 11:34 AM, "Bain,Virginia" <VEBain <@t> mdanderson.org> wrote:

>	Our lab recently got a new cryostat (a cryostar NX50).  It sees light to
>moderate usage (3 - 20 hours a week) and has been operational for about 3
>weeks.  It has had a host of problems since we received it (shipped with a
>busted motor and the sensor in the window did not detect when it was shut
>which lead to some kind of short and killed the lighting system).  I am
>hesitant to contact the rep again (they¹ve told us they¹re tired of
>hearing from us) until I¹m certain this is not user error of some sort.
>	For the most part we have been getting nice clean sections.  Every so
>often we start getting lines and tears which continue even when the blade
>is moved or replaced.  Twice now we have resolved this issue by rotating
>the anti-roll plate to a smooth edge.  When we encounter this problem I
>find that the anti-roll plate is jagged to the touch, which is I think
>what is causing the tears.  I see that some people suggest smoothing the
>anti-roll plate out on an emory board when it gets rough but this seems
>very early in the life of the anti-roll plate to me.  In my previous lab
>we used the same anti-roll plate without issue for about 2 years with
>heavy usage (50-70 hours a week) and so I¹m wondering why this anti-roll
>plate is encountering so much damage?
>Thanks for the help!
>Virginia Bain
>Postdoctoral Fellow
>Richie Lab
>M D Anderson - Science Park
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu

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