[Histonet] HA-tag antibody

[Anatoli Gleiberman]

HI Eva,
Goat anti-HA antibody from Abcam supposed to work on FFPE sections: http://www.abcam.com/ha-tag-antibody-ab94918.html
Don't know working dilution.
Rabbit antibody from Abcam should probably work as well - I tried them only on formalin-fixed cryo-sections: http://www.abcam.com/ha-tag-antibody-chip-grade-ab9110.html
I used it in dilution 1:200 for immunofluorescence.
Chicken anti-HA antibody from Aves (cat.#ET-HA100) should work as well: http://www.aveslab.com/products/epitope-tag-and-gfp-antibodies/anti-ha-hemagglutinin-antibodies/
Should work in dilution 1:500-1:1000 for immunofluorescence.

Anatoli Gleiberman, Ph.D.
Director of Histopathology
Buffalo Biolabs LLC
73 High Street
Buffalo, NY 14203
Phone: 716-849-6810x354
e-mail: agleiberman <@t> buffalobiolabs.com


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Eva Permaul
Sent: Wednesday, May 21, 2014 9:54 AM
To: histonet
Subject: [Histonet] HA-tag antibody

Hello,
I am looking for an HA-tag antibody to detect a protein labeled with HA in formalin fixed paraffin embedded tissues. Could anyone suggest a published antibody? The tissue of interest is mouse so I would prefer an antibody made in another species.
I have found several antibodies but can't find them published.
Thank you,
Eva
Georgetown University
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