[Histonet] HTL
Weems, Joyce K.
Joyce.Weems <@t> emoryhealthcare.org
Wed May 21 07:25:49 CDT 2014
Also the booklets from NSH ..
Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org
www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.
-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of madary <@t> verizon.net
Sent: Wednesday, May 21, 2014 6:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HTL
&nb=;WHen I took mine i did both parts. for the written carson
cover to cover=d the thick bancroft and stevens(sp?) 2 times cover
to cover.
Nick(Rocky) Madar= HT/HTL(ASCP)QIHC
Joni Madary, PhD(in life)
<=v style="border-top:1px solid #bcbcbc;margin:5px 0px;">
On 05/20/14, hist=et-request <@t> lists.utsouthwestern.edu wrote:
Send Histonet =iling list submissions to
[1]histonet <@t> lists.utsout=estern.edu
To subscribe or unsubscribe via the World Wide =b, visit
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or, via email, send a message =th subject or body 'help' to
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When replying, please edit your Subject line = it is more specific
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Today's Topics:
1. HTL certification (C�y Stan)
2. RE: HTL certification (Esther C Peters)
3. pos=ive control indoleamine 2-3 dioxygenase
(Pathology-Histology S= Supervisor)
4. immunohistochemisty coding (Michael LaFriniere) 5. antibody vials
(Clare Thornton)
6. RE: antibody vials (Mu=hy, Valerie)
7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks=apriya)
8. new processor (anita)
9. RE: antibody vials (A=e Murvosh)
10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da=hnapriya)
11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak=napriya)
----------------------------------------------- -----------------------
Message: 1
Date: Mon, 19 May 2014 1=32:36 -0500
From: Cecy Stan <[5]cecystan76 <@t> gmail.com>
Subject: [Histonet] HTL certification
To: [6]histone=lists.utsouthwestern.edu
Message-ID:
<CACh=0wgaaEfV DFmsuB-Jw8OKo=LKUP[7]ApGujjkoEAgjAzASxo9g <@t> mail.gmail.com=
Content-Type: text/plain; charset=UTF-8
Hello ev=yone,
I'm starting to prepare for my HTL certification (I am v=y nervous
and anxious but also very excited about this decision to g=or it).
I was just curious to know how you guys prepared for =, and how
long it
took for you to prepare before taking the test. Wi= 6 months
preparation
be enough? (I know that may depend on the indi=dual; it's just that
I
had my Masters over 10 years ago and I haven'=tudied this much
since
then).
I have Freida L. Carson's =d Edition book -- quite daunting to
memorize --
but the outline ASCP=s provided for study seems to be helpful. Do
you
have any other bo=/study aid suggestions?
Thank you in advance for your input an�dvice!
C.A.
------------------------------
Message: 2
Date: Mon, 19 May 2014 17:46:47 +0000
From: Esther=eters <[8]epeters2 <@t> gmu.edu>
Subject: RE: [Histonet] HTL ce=ification
To: Cecy Stan <[9]cecystan76 <@t> gmail.com>,
=9"[10]histonet <@t> lists.utsouthwestern.edu"
<[11]histonet <@t> lists.utsouthwestern.edu>
Me=age-ID: <[12]1400521609188.54980 <@t> gmu.edu>
Content-=pe: text/plain; charset="iso-8859-1"
Hi C.A.,
I don't have the HT or HTL, but from my college teaching experience in histology and histotechniques, I just wanted to caution you that
memoriz=g is not what you should be doing. You need to understand
concepts, so th= when you need to troubleshoot problems you will be
able to think through=ings, rule some things out, and make sense of
the situation. I see this =l the time with my students, they forget
things they memorize, but then t=y finally understand things and
can figure things out. One of the new tea=ing tools is having
students prepare "concept maps," to see the=lationships of topics
and terms, and these linkages will help you in the=ng run. For
histology examples, see:
[13]http://www.biologycorner.com/anatomy/tissues/tissue_conce pt_map_samples.html
I don't know of any concept maps for his=technology on the web, but
I am going to add this to my course next year! Esther
Esther C. Peters, Ph.D.
Assistant Profes=r
Environmental Science & Policy
George Mason University
4400 University Drive, MS 5F2
Fairfax, VA 22030-4444
Office: D=id King Hall, Room 3050
Phone: 703-993-3462
Fax: 703-993-1066e-mail: [14]epeters2 <@t> gmu.edu
[15]https://bluprd0511.outlook.com/owa/redir.aspx?C =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr
rDjhw=k.&URL=http%3a%2f%2fesp.gmu.edu
______________=________________________
From: [16]histonet-bo=ces <@t> lists.utsouthwestern.edu <[17]histonet-b ounces <@t> lists.utsouthwestern.edu> on behalf of Cecy Stan <[18]cec ystan76 <@t> gmail.com>
Sent: Monday, May 19, 2014 1:32 PM
To: =9]histonet <@t> lists.utsouthwestern.edu
Subject: [Hist=et] HTL certification
Hello everyone,
I'm starting = prepare for my HTL certification (I am very nervous
and anxious but=so very excited about this decision to go for it).
I was just=rious to know how you guys prepared for it, and how long
it
took fo=ou to prepare before taking the test. Will 6 months
preparation
be =ough? (I know that may depend on the individual; it's just that
I
ha=y Masters over 10 years ago and I haven't studied this much
since
t=n).
I have Freida L. Carson's 3rd Edition book -- quite daunti= to
memorize --
but the outline ASCP has provided for study seems t=e helpful. Do
you
have any other book/study aid suggestions?
<= />Thank you in advance for your input and advice!
C.A.
_______=______________________________________
Histonet mailing list
[20]Histonet <@t> lists.utsouthwestern.edu
[21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------
Messa=: 3
Date: Tue, 20 May 2014 12:09:28 +0000
From: "Pathology=istology Sr. Supervisor" <[22]histo <@t> skm.org.pk>
Subject:=istonet] positive control indoleamine 2-3 dioxygenase
To: "[23]histonet <@t> lists.utsouthwestern.edu"
<[24]histonet <@t> lists.utsouthwestern.edu>
Cc: "[25]tahseen0009 <@t> gmail.com" <[26]tahseen0009 <@t> gmail.com>Message-ID:
<BBFD4ECF5CBFAE4E936663D�B7AA8E62B266171 <@t> exchmbx.skm.org.pk>
Content-Type: text/plain;=arset="us-ascii"
Hi All,
We are going to opt=ize indoleamine 2-3 dioxygenase (IDO1)
expression in breast cancer tissue= immunohistochemistry on
formalin fixed paraffin sections.
I shall � thankful if any one please let me know about the
"positive control=
Regards
Muhammad Tahseen
Sr.Supervisor Histopathology
SKMCH&RC Lahore
Pakistan
-----------------------=-----
Message: 4
Date: Tue, 20 May 2014 13:03:55 +0000
From: Michael LaFriniere <[27]Michael.LaFriniere <@t> ccplab.com=
Subject: [Histonet] immunohistochemisty coding
To: "=[28]Sarah.Dysart <@t> stdavids.com'" <[29]Sarah.Dysart @stdavids.com>,
"[30]histonet <@t> lists.utsou=western.edu"
<[31]histonet <@t> lists.utsout=estern.edu>
Message-ID:
<4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90 <@t> AHCMSASEXCH02.my.ahc.lo
cal>
Content-Type: text/plain; charset="us-ascii"
Hi Sarah,
Joyce is correct, under medicare changes in patho=gy coding, you
can not bill for each of the blocks of the same antibody a pplications, only one charge per specific antibody mentioned in the
patholo= report is allowable. Medicare immuno billing is per
"specimen"=ly for each specific antibody used, example if you used
s100 for the sam=pecimen (wide melanoma excision)on 15 different
blocks, under medicare y= can only bill one GO461, yes- not fair
when the need is appropriate,
I must voice my opinio)- this probably will lower some of the over utilization and inappropriate billing practices that many of us have
seen = the immunohistochemistry world and the labs that have
capitalized on the=actice, the problem with the lower
reimbursements and change in medicare=ding hurts many of our labs
who have used the utilization of immunohisto=emistry appropriately.
However, "under current 2014&=ot; CPT coding on non medicare can
you bill for the additional work per b=ck using the 88342 and 88343
cpt code, I am sure moving forward we are go=g to continue to see
adjustments to the pathology coding processes!
Michael R. LaFriniere, HT (ASCP)
Executive Director
Capital Choice Pathology Laboratory
12041 Bournefield Way, Suit� * Silver Spring, MD 20904
P: 240.471.3427 * F: 240.471.3401 * Ce= 410-940-8844
[33]michael.lafriniere <@t> CCPLab.com
-----Original Message-----
From: [34]histonet-bounces <@t> lists.utsouthwestern.edu [ma ilto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Weems,
Joy� K.
Sent: Monday, May 19, 2014 12:21 PM
To: '[35]Sarah.Dy=rt <@t> stdavids.com';
[36]histonet <@t> lists.utsouthwestern.edu Subject: [Histonet] RE: Billing
No - one G0461.
<= />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1
and G0462=. j
Joyce Weems
Pathology Manager
678-843-7376 Pho=
678-843-7831 Fax
[37]joyce.weems <@t> emoryhealthcare.o=
[38]www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342
This e-ma=, including any attachments is the property of Saint
Joseph's Hospital an=s intended for the sole use of the intended
recipient(s). It may contai=nformation that is privileged and
confidential. Any unauthorized review=se, disclosure, or
distribution is prohibited. If you are not the intend� recipient,
please delete this message, and reply to the sender regarding=e
error in a separate email.
-----Original Message-----
=om: [39]histonet-bounces <@t> lists.utsouthwestern.edu
[[40]mailto:histonet-bounces <@t> lists.utsouthwestern.e=] On Behalf Of
[41]Sarah.Dysart <@t> stdavids.com
Sent: Mon�y, May 19, 2014 12:00 PM
To: [42]histonet <@t> lists.utsout=estern.edu
Subject: [Histonet] Billing
When it comes = the medicare codes...question...
So you have one specimen tha=as 10 blocks.
AE1/AE3 is ordered on all 10 blocks.
Can you bil=E1/AE3 1st Antibody once (G0461), then AE1/AE3
Additional (G0462) nine t=es?
Thanks,
Sarah E. Dysart, BA, HT (ASCP), QIHC (A=P) Pathology Supervisor St.
David's North Austin Medical Center
1222=orth Mopac Expressway
Austin, Texas 78758
(512)901-1220
_______________________________________________
Histonet mailing=st
[43]Histonet <@t> lists.utsouthwestern.edu
[44]http://lists.utsouthwestern.edu/mailman/list=fo/histonet
________________________________
Th= e-mail message (including any attachments) is for the sole use
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privileged
i=ormation. If the reader of this message is not the intended
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[46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<= />
------------------------------
Message: 5
Date: =e, 20 May 2014 10:48:20 -0400
From: Clare Thornton <[47]CThor=on <@t> dahlchase.com>
Subject: [Histonet] antibody vials
To:=48]histonet <@t> lists.utsouthwestern.edu"
=9<[49]histonet <@t> lists.utsouthwestern.edu>
Mes=ge-ID:
<C9D78FFC9D668B4CBEA4405F[50]84697 504013D73DEB4F1 <@t> iris.dahlchase.net>
Content-Type: text/plain; c=rset="us-ascii"
Does anyone have any good ideas fo=eeping the little vials of
concentrated antibodies neat and organized in=e fridge? Right now
we keep ours in empty pipette tip boxes (without ti=olders) but
they're always falling over and mixed up. We use several di�erent
vendors for our concentrated antibodies, so our vials are all diffe rent sizes. Thanks for any ideas!
Clare J. Thornton=TL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Dahl-Chase=agnostic Services
417 State Street, Suite 540
Bangor, ME 04401 [51]cthornton <@t> dahlchase.com<[52]mailto:cthornton <@t> dahl=ase.com>
----------------------------=
Message: 6
Date: Tue, 20 May 2014 14:59:21 +0000
Fr=: "Murphy, Valerie" <[53]murphyv <@t> karmanos.org>
=bject: [Histonet] RE: antibody vials
To: Clare Thornton <[54]=hornton <@t> dahlchase.com>,
"histonet <@t> l=ts.utsouthwestern.edu"
<[55]histonet <@t> li=s.utsouthwestern.edu>
Message-ID:
<A0CD799E6FCD494�AD4[56]A25377936712AE1BB3CB <@t> EX-MB2.kci-net.k armanos.org>
Content-Type: text/plain; charset="us-ascii=
I use cardboard cryovial boxes and they work quite well. =u can
remove some of the dividers to make space for the larger vials.
[57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre
eze=Racks/Cryovial-Boxes-Cardboard/
Valerie =tliff B.Sc HTL(ASCP)
Research Assistant
Department= Oncology
Karmanos Cancer Institute
4100 John R
Detroit, MI 48201
Telephone: (313) 576 8282 Fax: (313) 576 8306
E-mail: [58]murphyv <@t> karmanos.or=
Better treatments. Better outcomes.
________________________________________
From: [59]histonet-bounces <@t> lists.utsouthwestern.edu
[[60]histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Clare
Thornto=[61]CThornton <@t> dahlchase.com]
Sent: Tuesday, May 20, 2014 1=48 AM
To: [62]histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] antibody vials
Does anyone have any good =eas for keeping the little vials of
concentrated antibodies neat and orga=zed in the fridge? Right now
we keep ours in empty pipette tip boxes (wi=out tip holders) but
they're always falling over and mixed up. We use se=ral different
vendors for our concentrated antibodies, so our vials are a=
different sizes. Thanks for any ideas!
Clare J. =ornton, HTL(ASCP)QIHC
Lead Immunohistochemistry Technologist
Da=-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, = 04401
[63]cthornton <@t> dahlchase.com<[64]mailto:cthornto=dahlchase.com>
__________________________________=___________
Histonet mailing list
[65]Histonet <@t> li=s.utsouthwestern.edu
[66]http://li=s.utsouthwestern.edu/mailman/listinfo/histonet
----------- Confidentiality Notice: This email message, including any attachments , is for the sole use of the intended recipient(s) and may contain
confiden=al and/or privileged information. If you are not the
intended recipient(s= you are hereby notified that any
dissemination, unauthorized review, use=isclosure or distribution
of this email and any materials contained in a= attachments is
prohibited. If you receive this message in error, or are =t the
intended recipient(s), please immediately notify the sender by emai=
and destroy all copies of the original message, including attachments.
------------------------------
Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT)
From: "Balasubbra=nian, Dakshnapriya"
<[67]dakshnapriya <@t> neo.tamu.edu><= />Subject: [Histonet] HELP-
Cryosectioning FAT!
To: [68]histonet <@t> lists.utsouthwestern.edu
Message-ID:
<21128R24.17352091.1400599[69]407431.JavaMail.root <@t> neo.tamu.edu>
Content-Type: text/plain; charset=utf-8
Hi all,
I've been having problems with cryosectioning fatty tissue for a lo ng time now. The section leaves a hole in the middle. I know this is
probab= a much discussed topic, but I've tried a lot of strategies
with no luck.=e tissue is of mouse origin and has micro-lesions
buried inside fat. So =e fat needs to be cut in order to get to the
lesion. I started with a tem=rature of -17C and tried upto -25C and
also at -50C; didn't work at any o=hese temperatures. I tried
rubbing the block, blade and anti-roll plate =th dry ice-again,no
luck.
I rapid-freeze the tissue with OCT = LN2 and then store the block
at -80C. Could I try sectioning the block d=ectly from -80C? (never
done that).
Any other suggestions on =w to tackle this?
Any advice is much appreciated!!
=anks,
Dakshna
------------------------------<= />
Message: 8
Date: Tue, 20 May 2014 10:26:13 -0500
From:=ita <[70]azdudley <@t> hotmail.com>
Subject: [Histonet] new p=cessor
To: "[71]Histonet <@t> lists.utsouthwestern.edu=
<[72]histonet <@t> lists.utsouthwestern.edu<=>>
Message-ID: <COL131-W4520B92[73]279A33E8D42CCF0DD0 <@t> phx.gbl>
Content-Type: text/plain; charset="iso-885=1"
Thanks everyone for your input on processors, I think= will stay
with the VIP, it has been a good machine just getting older n=.
everyone have a great day!!!!
Anita Dudley
Providence Hosp
Mobile, Alabama 3f95
------------------------------<= />
Message: 9
Date: Tue, 20 May 2014 08:29:05 -0700
From:=nne Murvosh" <[74]amurvosh <@t> advancederm.net>
Subject: RE: [Histonet] antibody vials
To: "Clare Thornton"=[75]CThornton <@t> dahlchase.com>,
<[76]histonet <@t> lists.utsouthwestern.edu>
Message-ID:
<4A�A4E531E8C943A7[77]30559B6B81DF07DA8970 <@t> dc.Advancederm=et>
Content-Type: text/plain; charset="us-ascii"=
We got some plexiglass dividers at the "Container Store&q=t; if you
have one
near or check online. Can't remember what they w=e called but they
had
different sized square compartments that fit v=ious venders
antibodies.
Anne
-----Original Message-----<= />From:
[78]histonet-bounces <@t> lists.utsouthwestern.�u
[[79]mailto:histonet-bounces <@t> lists.utsout=estern.edu] On Behalf Of
Clare
Thornton
Sent: Tuesday, May , 2014 7:48 AM
To: [80]histonet <@t> lists.utsouthwestern.e=
Subject: [Histonet] antibody vials
Does anyone have =y good ideas for keeping the little vials of
concentrated antibodies=at and organized in the fridge? Right now
we
keep ours in empty pi=tte tip boxes (without tip holders) but
they're
always falling over =d mixed up. We use several different vendors
for
our concentrated a=ibodies, so our vials are all different sizes.
Thanks for any ideas! Clare J. Thornton, HTL(ASCP)QIHC
Lead Immunohi=ochemistry Technologist
Dahl-Chase Diagnostic Services
417 Stat=treet, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.=m<[81]mailto:cthornton <@t> dahlchase.com>
_=____________________________________________
Histonet mailing list[82]Histonet <@t> lists.utsouthwestern.edu
[83]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet
------------------------------
Message: 10
Date: Tue, 20 May 2014 10:59:16 -0500 (CDT)
From: =alasubbramanian, Dakshnapriya" <dakshnapriya <@t> neo.t=u.edu>
Subject: Re: [Histonet] HELP- Cryosectioning FAT!
To: "Sandra E. Esparza" <[84]SEsparza <@t> seton.org>,
=9[85]histonet <@t> lists.utsouthwestern.edu
Message-ID: <601376475.17568918.1400601[86]556617.JavaMail.ro=@neo.tamu.edu>
Content-Type: text/plain; charset=utf-8
Thanks Sandra! My next step was to try that.
Dakshna
----- Original Message -----
From: "Sandra E. Esparza"=[87]SEsparza <@t> seton.org>
To: "Dakshnapriya Balasubbram=ian" <[88]dakshnapriya <@t> neo.tamu.edu>
Sent: Tues�y, May 20, 2014 10:34:25 AM
Subject: RE: [Histonet] HELP- Cryosectio=ng FAT!
You might want to dry Liquid Nitrogen on the face of t= block
before you cut it.
Sandra
Sandra Esparza H=ASCP), QIHC
Histotechnologist Mohs
Austin Dermatologic Surgery�nter
512-324-7468 x84027
[89]sesparza <@t> seton.org
-----Original Message-----
From: [90]his=net-bounces <@t> lists.utsouthwestern.edu [[91]mailto :histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Balasubbramani=, Dakshnapriya
Sent: Tuesday, May 20, 2014 10:23 AM
To: [92]histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] =LP- Cryosectioning FAT!
Hi all,
I've been having pr=lems with cryosectioning fatty tissue for a
long time now. The section le=es a hole in the middle. I know this
is probably a much discussed topic, =t I've tried a lot of
strategies with no luck. The tissue is of mouse ori=n and has
micro-lesions buried inside fat. So the fat needs to be cut in =der
to get to the lesion. I started with a temperature of -17C and tried upto -25C and also at -50C; didn't work at any of these temperatures.
I tri� rubbing the block, blade and anti-roll plate with dry
ice-again,no luck. I rapid-freeze the tissue with OCT in LN2 and then store the b=ck
at -80C. Could I try sectioning the block directly from -80C? (never
d=e that).
Any other suggestions on how to tackle this?
Any advice is much appreciated!!
Thanks,
Dakshna
<= />_______________________________________________
Histonet mailing =st
[93]Histonet <@t> lists.utsouthwestern.edu
[94]http://lists.utsouthwestern.edu/mailman/listi=o/histonet
CONFIDENTIALITY NOTICE:
This email m=sage and any accompanying data or files is
confidential and may contain p=vileged information intended only
for the named recipient(s). If you are =t the intended
recipient(s), you are hereby notified that the disseminati=,
distribution, and or copying of this message is strictly prohibited.
If=u receive this message in error, or are not the named
recipient(s), plea= notify the sender at the email address above,
delete this email from you=omputer, and destroy any copies in any
form immediately. Receipt by anyo= other than the named
recipient(s) is not a waiver of any attorney-client=ork product,
or other applicable privilege.
-----=-----------------------
Message: 11
Date: Tue, 20 May 201A1:03:08 -0500 (CDT)
From: "Balasubbramanian, Dakshnapriya"=
<[95]dakshnapriya <@t> neo.tamu.edu>
Subject: Re: [Histon=] HELP- Cryosectioning FAT!
To: Laurie J King <[96]k=g.laurie <@t> marshfieldclinic.org>,
histonet=ists.utsouthwestern.edu
Message-ID:
<262036458.175957e.1400601[97]788819.JavaMail.root <@t> neo.tamu.edu>
=ntent-Type: text/plain; charset=utf-8
Hi Laurie,
=, the tissue hasn't been fixed before freezing. My prof doesn't
prefer th=ethod, but if nothing else works, we might give it a
try.
An=uggested protocols for fixation?
Thanks!
Dakshna
----- Original Message -----
From: "Laurie J King" <<�lass="parsedEmail"
href="mailto:king.laurie <@t> marshfieldclinic.org" ta rget="_blank">king.laurie <@t> marshfieldclinic.org>
To: "Dak=napriya Balasubbramanian" <[98]dakshnapriya <@t> neo.tamu.edu>
Sent: Tuesday, May 20, 2014 10:37:35 AM
Subject: RE: [Histo=t] HELP- Cryosectioning FAT!
Dakshna,
Has this tiss= been fixed before freezing by any chance?
laurie
-=--Original Message-----
From: [99]histonet-bou=es <@t> lists.utsouthwestern.edu
[[100]mailto:histonet=ounces <@t> lists.utsouthwestern.edu] On Behalf Of
Balasubbramanian, Daksh=priya
Sent: Tuesday, May 20, 2014 10:23 AM
To: [101]histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HELP- Cryo=ctioning FAT!
Hi all,
I've been having problems wit=ryosectioning fatty tissue for a
long time now. The section leaves a hol=n the middle. I know this
is probably a much discussed topic, but I've t=ed a lot of
strategies with no luck. The tissue is of mouse origin and ha=
micro-lesions buried inside fat. So the fat needs to be cut in order
to g= to the lesion. I started with a temperature of -17C and tried
upto -25C =d also at -50C; didn't work at any of these
temperatures. I tried rubbing=e block, blade and anti-roll plate
with dry ice-again,no luck.
I rapid-freeze the tissue with OCT in LN2 and then store the block at
-8
. Could I try sectioning the block directly from -80C? (never
done that). Any other suggestions on how to tackle this?
Any ad=ce is much appreciated!!
Thanks,
Dakshna
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