[Histonet] HTL

Weems, Joyce K. Joyce.Weems <@t> emoryhealthcare.org
Wed May 21 07:25:49 CDT 2014


Also the booklets from NSH ..

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.weems <@t> emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

This e-mail, including any attachments is the property of Saint Joseph’s Hospital and is intended for the sole use of the intended recipient(s).  It may contain information that is privileged and confidential.  Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please delete this message, and reply to the sender regarding the error in a separate email.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of madary <@t> verizon.net
Sent: Wednesday, May 21, 2014 6:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] HTL


   &nb=;WHen  I  took  mine  i did both parts. for the written carson
   cover  to cover=d the thick bancroft and stevens(sp?) 2 times cover
   to cover.



   Nick(Rocky) Madar= HT/HTL(ASCP)QIHC
   Joni Madary, PhD(in life)

   <=v style="border-top:1px solid #bcbcbc;margin:5px 0px;">

   On 05/20/14, hist=et-request <@t> lists.utsouthwestern.edu wrote:




   Send Histonet =iling list submissions to
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   Today's Topics:
   1. HTL certification (C�y Stan)
   2. RE: HTL certification (Esther C Peters)
   3. pos=ive control indoleamine 2-3 dioxygenase
   (Pathology-Histology S= Supervisor)
   4.  immunohistochemisty  coding (Michael LaFriniere) 5. antibody vials
   (Clare Thornton)
   6. RE: antibody vials (Mu=hy, Valerie)
   7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks=apriya)
   8. new processor (anita)
   9. RE: antibody vials (A=e Murvosh)
   10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da=hnapriya)
   11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak=napriya)
   -----------------------------------------------   -----------------------
   Message: 1
   Date: Mon, 19 May 2014 1=32:36 -0500
   From: Cecy Stan <[5]cecystan76 <@t> gmail.com>
   Subject: [Histonet] HTL certification
   To: [6]histone=lists.utsouthwestern.edu
   Message-ID:
        <CACh=0wgaaEfV   DFmsuB-Jw8OKo=LKUP[7]ApGujjkoEAgjAzASxo9g <@t> mail.gmail.com=
   Content-Type: text/plain; charset=UTF-8
   Hello ev=yone,
   I'm starting to prepare for my HTL certification (I am v=y nervous
   and anxious but also very excited about this decision to g=or it).
   I  was  just  curious  to know how you guys prepared for =, and how
   long it
   took  for  you  to  prepare  before  taking  the test. Wi= 6 months
   preparation
   be enough? (I know that may depend on the indi=dual; it's just that
   I
   had  my  Masters  over  10 years ago and I haven'=tudied this much
   since
   then).
   I  have  Freida  L.  Carson's  =d Edition book -- quite daunting to
   memorize --
   but  the  outline ASCP=s provided for study seems to be helpful. Do
   you
   have any other bo=/study aid suggestions?
   Thank you in advance for your input an�dvice!
   C.A.
   ------------------------------
   Message: 2
   Date: Mon, 19 May 2014 17:46:47 +0000
   From: Esther=eters <[8]epeters2 <@t> gmu.edu>
   Subject: RE: [Histonet] HTL ce=ification
   To: Cecy Stan <[9]cecystan76 <@t> gmail.com>,
   =9"[10]histonet <@t> lists.utsouthwestern.edu"
        <[11]histonet <@t> lists.utsouthwestern.edu>
   Me=age-ID: <[12]1400521609188.54980 <@t> gmu.edu>
   Content-=pe: text/plain; charset="iso-8859-1"
   Hi C.A.,
   I  don't have the HT or HTL, but from my college teaching experience    in  histology  and  histotechniques, I just wanted to caution you that
   memoriz=g  is not what you should be doing. You need to understand
   concepts, so th= when you need to troubleshoot problems you will be
   able to think through=ings, rule some things out, and make sense of
   the situation. I see this =l the time with my students, they forget
   things  they  memorize,  but then t=y finally understand things and
   can  figure  things  out.  One  of  the new tea=ing tools is having
   students  prepare  "concept maps," to see the=lationships of topics
   and  terms,  and  these  linkages  will help you in the=ng run. For
   histology examples, see:
   [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce   pt_map_samples.html
   I don't know of any concept maps for his=technology on the web, but
   I am going to add this to my course next year!   Esther
   Esther C. Peters, Ph.D.
   Assistant Profes=r
   Environmental Science & Policy
   George Mason University
   4400 University Drive, MS 5F2
   Fairfax, VA 22030-4444
   Office: D=id King Hall, Room 3050
   Phone: 703-993-3462
   Fax: 703-993-1066e-mail: [14]epeters2 <@t> gmu.edu
   [15]https://bluprd0511.outlook.com/owa/redir.aspx?C   =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr
   rDjhw=k.&URL=http%3a%2f%2fesp.gmu.edu
   ______________=________________________
   From: [16]histonet-bo=ces <@t> lists.utsouthwestern.edu <[17]histonet-b   ounces <@t> lists.utsouthwestern.edu>  on  behalf  of  Cecy  Stan <[18]cec   ystan76 <@t> gmail.com>
   Sent: Monday, May 19, 2014 1:32 PM
   To: =9]histonet <@t> lists.utsouthwestern.edu
   Subject: [Hist=et] HTL certification
   Hello everyone,
   I'm starting = prepare for my HTL certification (I am very nervous
   and anxious but=so very excited about this decision to go for it).
   I was just=rious to know how you guys prepared for it, and how long
   it
   took  fo=ou  to  prepare  before  taking the test. Will 6 months
   preparation
   be =ough? (I know that may depend on the individual; it's just that
   I
   ha=y  Masters  over 10 years ago and I haven't studied this much
   since
   t=n).
   I  have  Freida  L.  Carson's  3rd Edition book -- quite daunti= to
   memorize --
   but  the outline ASCP has provided for study seems t=e helpful. Do
   you
   have any other book/study aid suggestions?
   <= />Thank you in advance for your input and advice!
   C.A.
   _______=______________________________________
   Histonet mailing list
   [20]Histonet <@t> lists.utsouthwestern.edu
   [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet   ------------------------------
   Messa=: 3
   Date: Tue, 20 May 2014 12:09:28 +0000
   From: "Pathology=istology Sr. Supervisor" <[22]histo <@t> skm.org.pk>
   Subject:=istonet] positive control indoleamine 2-3 dioxygenase
   To: "[23]histonet <@t> lists.utsouthwestern.edu"
        <[24]histonet <@t> lists.utsouthwestern.edu>
   Cc: "[25]tahseen0009 <@t> gmail.com" <[26]tahseen0009 <@t> gmail.com>Message-ID:
        <BBFD4ECF5CBFAE4E936663D�B7AA8E62B266171 <@t> exchmbx.skm.org.pk>
   Content-Type: text/plain;=arset="us-ascii"
   Hi All,
   We  are  going  to  opt=ize  indoleamine  2-3  dioxygenase  (IDO1)
   expression   in  breast  cancer  tissue=  immunohistochemistry  on
   formalin fixed paraffin sections.
   I  shall  �  thankful  if  any  one  please  let me know about the
   "positive control=
   Regards
   Muhammad Tahseen
   Sr.Supervisor Histopathology
   SKMCH&RC Lahore
   Pakistan
   -----------------------=-----
   Message: 4
   Date: Tue, 20 May 2014 13:03:55 +0000
   From: Michael LaFriniere <[27]Michael.LaFriniere <@t> ccplab.com=
   Subject: [Histonet] immunohistochemisty coding
   To:     "=[28]Sarah.Dysart <@t> stdavids.com'"    <[29]Sarah.Dysart   @stdavids.com>,
        "[30]histonet <@t> lists.utsou=western.edu"
        <[31]histonet <@t> lists.utsout=estern.edu>
   Message-ID:
        <4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90 <@t> AHCMSASEXCH02.my.ahc.lo
   cal>
   Content-Type: text/plain; charset="us-ascii"
   Hi Sarah,
   Joyce  is  correct,  under medicare changes in patho=gy coding, you
   can  not  bill  for  each  of  the  blocks  of  the  same  antibody a   pplications,  only  one  charge per specific antibody mentioned in the
   patholo=  report  is  allowable.  Medicare  immuno  billing is per
   "specimen"=ly for each specific antibody used, example if you used
   s100  for  the sam=pecimen (wide melanoma excision)on 15 different
   blocks,  under  medicare  y= can only bill one GO461, yes- not fair
   when the need is appropriate,
   I  must  voice  my opinio)- this probably will lower some of the over   utilization  and  inappropriate billing practices that many of us have
   seen  =  the  immunohistochemistry  world  and  the labs that have
   capitalized   on   the=actice,   the   problem   with  the  lower
   reimbursements  and  change in medicare=ding hurts many of our labs
   who have used the utilization of immunohisto=emistry appropriately.
   However,  "under  current  2014&=ot; CPT coding on non medicare can
   you bill for the additional work per b=ck using the 88342 and 88343
   cpt  code,  I am sure moving forward we are go=g to continue to see
   adjustments to the pathology coding processes!
      Michael R. LaFriniere, HT (ASCP)
   Executive Director
   Capital Choice Pathology Laboratory
   12041 Bournefield Way, Suit� * Silver Spring, MD 20904
   P: 240.471.3427 * F: 240.471.3401 * Ce= 410-940-8844
   [33]michael.lafriniere <@t> CCPLab.com
      -----Original Message-----
   From:        [34]histonet-bounces <@t> lists.utsouthwestern.edu        [ma   ilto:histonet-bounces <@t> lists.utsouthwestern.edu]  On  Behalf  Of Weems,
   Joy� K.
   Sent: Monday, May 19, 2014 12:21 PM
   To: '[35]Sarah.Dy=rt <@t> stdavids.com';
   [36]histonet <@t> lists.utsouthwestern.edu   Subject: [Histonet] RE: Billing
   No - one G0461.
   <=  />If you had AE1/AE3, Melan A, S100 you would charge G0461 x 1
   and G0462=. j
   Joyce Weems
   Pathology Manager
   678-843-7376 Pho=
   678-843-7831 Fax
   [37]joyce.weems <@t> emoryhealthcare.o=
   [38]www.saintjosephsatlanta.org
   5665 Peachtree Dunwoody Road
   Atlanta, GA 30342
   This  e-ma=,  including  any  attachments is the property of Saint
   Joseph's  Hospital  an=s intended for the sole use of the intended
   recipient(s).  It  may  contai=nformation that is privileged and
   confidential.   Any   unauthorized   review=se,  disclosure,  or
   distribution  is  prohibited. If you are not the intend� recipient,
   please  delete  this  message,  and reply to the sender regarding=e
   error in a separate email.
   -----Original Message-----
   =om:         [39]histonet-bounces <@t> lists.utsouthwestern.edu
   [[40]mailto:histonet-bounces <@t> lists.utsouthwestern.e=] On Behalf Of
   [41]Sarah.Dysart <@t> stdavids.com
   Sent: Mon�y, May 19, 2014 12:00 PM
   To: [42]histonet <@t> lists.utsout=estern.edu
   Subject: [Histonet] Billing
   When it comes = the medicare codes...question...
   So you have one specimen tha=as 10 blocks.
   AE1/AE3 is ordered on all 10 blocks.
   Can  you  bil=E1/AE3  1st  Antibody  once  (G0461), then AE1/AE3
   Additional (G0462) nine t=es?
   Thanks,
   Sarah E. Dysart, BA, HT (ASCP), QIHC (A=P) Pathology Supervisor St.
   David's North Austin Medical Center
   1222=orth Mopac Expressway
   Austin, Texas 78758
   (512)901-1220
      _______________________________________________
   Histonet mailing=st
   [43]Histonet <@t> lists.utsouthwestern.edu
   [44]http://lists.utsouthwestern.edu/mailman/list=fo/histonet
   ________________________________
   Th= e-mail message (including any attachments) is for the sole use
   of
   t=   intended  recipient(s)  and  may  contain  confidential  and
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   i=ormation. If the reader of this message is not the intended
   recipien=   you  are  hereby  notified  that  any  dissemination,
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   [46]http://lists.utsouthwestern.edu/mailman/listinfo/histonet
   <= />
   ------------------------------
   Message: 5
   Date: =e, 20 May 2014 10:48:20 -0400
   From: Clare Thornton <[47]CThor=on <@t> dahlchase.com>
   Subject: [Histonet] antibody vials
   To:=48]histonet <@t> lists.utsouthwestern.edu"
   =9<[49]histonet <@t> lists.utsouthwestern.edu>
   Mes=ge-ID:
        <C9D78FFC9D668B4CBEA4405F[50]84697   504013D73DEB4F1 <@t> iris.dahlchase.net>
   Content-Type: text/plain; c=rset="us-ascii"
   Does  anyone  have  any  good  ideas fo=eeping the little vials of
   concentrated  antibodies  neat and organized in=e fridge? Right now
   we  keep  ours  in empty pipette tip boxes (without ti=olders) but
   they're  always  falling over and mixed up. We use several di�erent
   vendors  for  our concentrated antibodies, so our vials are all diffe   rent sizes. Thanks for any ideas!
   Clare J. Thornton=TL(ASCP)QIHC
   Lead Immunohistochemistry Technologist
   Dahl-Chase=agnostic Services
   417 State Street, Suite 540
   Bangor, ME 04401   [51]cthornton <@t> dahlchase.com<[52]mailto:cthornton <@t> dahl=ase.com>
   ----------------------------=
   Message: 6
   Date: Tue, 20 May 2014 14:59:21 +0000
   Fr=: "Murphy, Valerie" <[53]murphyv <@t> karmanos.org>
   =bject: [Histonet] RE: antibody vials
   To: Clare Thornton <[54]=hornton <@t> dahlchase.com>,
        "histonet <@t> l=ts.utsouthwestern.edu"
        <[55]histonet <@t> li=s.utsouthwestern.edu>
   Message-ID:
        <A0CD799E6FCD494�AD4[56]A25377936712AE1BB3CB <@t> EX-MB2.kci-net.k   armanos.org>
   Content-Type: text/plain; charset="us-ascii=
   I  use  cardboard  cryovial  boxes and they work quite well. =u can
   remove some of the dividers to make space for the larger vials.
   [57]http://labscientific.com/Cryogenic-Supplies/Cryovial-Boxes-and-Fre
   eze=Racks/Cryovial-Boxes-Cardboard/
   Valerie =tliff B.Sc HTL(ASCP)
   Research Assistant
   Department= Oncology
   Karmanos Cancer Institute
   4100 John R
   Detroit, MI 48201
   Telephone: (313) 576 8282   Fax: (313) 576 8306
   E-mail: [58]murphyv <@t> karmanos.or=
   Better treatments. Better outcomes.
   ________________________________________
   From:                    [59]histonet-bounces <@t> lists.utsouthwestern.edu
   [[60]histonet-bounces <@t> lists.utsouthwestern.edu]  on  behalf  of  Clare
   Thornto=[61]CThornton <@t> dahlchase.com]
   Sent: Tuesday, May 20, 2014 1=48 AM
   To: [62]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] antibody vials
   Does  anyone  have  any  good  =eas for keeping the little vials of
   concentrated  antibodies neat and orga=zed in the fridge? Right now
   we  keep  ours  in empty pipette tip boxes (wi=out tip holders) but
   they're  always  falling over and mixed up. We use se=ral different
   vendors  for  our  concentrated  antibodies,  so  our  vials are a=
   different sizes. Thanks for any ideas!
   Clare J. =ornton, HTL(ASCP)QIHC
   Lead Immunohistochemistry Technologist
   Da=-Chase Diagnostic Services
   417 State Street, Suite 540
   Bangor, = 04401
   [63]cthornton <@t> dahlchase.com<[64]mailto:cthornto=dahlchase.com>
   __________________________________=___________
   Histonet mailing list
   [65]Histonet <@t> li=s.utsouthwestern.edu
   [66]http://li=s.utsouthwestern.edu/mailman/listinfo/histonet
   -----------   Confidentiality Notice: This email message, including any attachments   ,  is  for  the  sole use of the intended recipient(s) and may contain
   confiden=al  and/or  privileged  information.  If  you are not the
   intended   recipient(s=   you   are   hereby  notified  that  any
   dissemination,  unauthorized review, use=isclosure or distribution
   of  this  email  and  any  materials contained in a= attachments is
   prohibited.  If  you  receive  this message in error, or are =t the
   intended recipient(s), please immediately notify the sender by emai=
   and destroy all copies of the original message, including attachments.
   ------------------------------
   Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT)
   From: "Balasubbra=nian, Dakshnapriya"
   <[67]dakshnapriya <@t> neo.tamu.edu><=   />Subject:  [Histonet]  HELP-
   Cryosectioning FAT!
   To: [68]histonet <@t> lists.utsouthwestern.edu
   Message-ID:
        <21128R24.17352091.1400599[69]407431.JavaMail.root <@t> neo.tamu.edu>
   Content-Type: text/plain; charset=utf-8
   Hi all,
   I've  been  having problems with cryosectioning fatty tissue for a lo   ng  time  now. The section leaves a hole in the middle. I know this is
   probab= a much discussed topic, but I've tried a lot of strategies
   with  no  luck.=e  tissue is of mouse origin and has micro-lesions
   buried inside fat. So =e fat needs to be cut in order to get to the
   lesion. I started with a tem=rature of -17C and tried upto -25C and
   also  at  -50C;  didn't  work  at any o=hese temperatures. I tried
   rubbing  the  block, blade and anti-roll plate =th dry ice-again,no
   luck.
   I  rapid-freeze  the tissue with OCT = LN2 and then store the block
   at -80C. Could I try sectioning the block d=ectly from -80C? (never
   done that).
   Any other suggestions on =w to tackle this?
   Any advice is much appreciated!!
   =anks,
   Dakshna
   ------------------------------<= />
   Message: 8
   Date: Tue, 20 May 2014 10:26:13 -0500
   From:=ita <[70]azdudley <@t> hotmail.com>
   Subject: [Histonet] new p=cessor
   To: "[71]Histonet <@t> lists.utsouthwestern.edu=
        <[72]histonet <@t> lists.utsouthwestern.edu<=>>
   Message-ID: <COL131-W4520B92[73]279A33E8D42CCF0DD0 <@t> phx.gbl>
   Content-Type: text/plain; charset="iso-885=1"
   Thanks  everyone  for  your input on processors, I think= will stay
   with the VIP, it has been a good machine just getting older n=.
   everyone have a great day!!!!
   Anita Dudley
   Providence Hosp
   Mobile, Alabama 3f95

   ------------------------------<= />
   Message: 9
   Date: Tue, 20 May 2014 08:29:05 -0700
   From:=nne Murvosh" <[74]amurvosh <@t> advancederm.net>
   Subject: RE: [Histonet] antibody vials
   To: "Clare Thornton"=[75]CThornton <@t> dahlchase.com>,
        <[76]histonet <@t> lists.utsouthwestern.edu>
   Message-ID:
        <4A�A4E531E8C943A7[77]30559B6B81DF07DA8970 <@t> dc.Advancederm=et>
   Content-Type: text/plain;    charset="us-ascii"=
   We got some plexiglass dividers at the "Container Store&q=t; if you
   have one
   near  or check online. Can't remember what they w=e called but they
   had
   different  sized  square  compartments  that  fit  v=ious  venders
   antibodies.
   Anne
   -----Original Message-----<= />From:
   [78]histonet-bounces <@t> lists.utsouthwestern.�u
   [[79]mailto:histonet-bounces <@t> lists.utsout=estern.edu] On Behalf Of
   Clare
   Thornton
   Sent: Tuesday, May  , 2014 7:48 AM
   To: [80]histonet <@t> lists.utsouthwestern.e=
   Subject: [Histonet] antibody vials
   Does anyone have =y good ideas for keeping the little vials of
   concentrated  antibodies=at and organized in the fridge? Right now
   we
   keep  ours  in  empty  pi=tte  tip boxes (without tip holders) but
   they're
   always  falling  over =d mixed up. We use several different vendors
   for
   our concentrated a=ibodies, so our vials are all different sizes.
   Thanks for any ideas!   Clare J. Thornton, HTL(ASCP)QIHC
   Lead Immunohi=ochemistry Technologist
   Dahl-Chase Diagnostic Services
   417 Stat=treet, Suite 540
   Bangor, ME 04401
   cthornton <@t> dahlchase.=m<[81]mailto:cthornton <@t> dahlchase.com>
   _=____________________________________________
   Histonet mailing list[82]Histonet <@t> lists.utsouthwestern.edu
   [83]http://lists.utsouthwestern.edu/mailman/listinfo/=stonet
   ------------------------------
   Message: 10
   Date: Tue, 20 May 2014 10:59:16 -0500 (CDT)
   From: =alasubbramanian, Dakshnapriya" <dakshnapriya <@t> neo.t=u.edu>
   Subject: Re: [Histonet] HELP- Cryosectioning FAT!
   To: "Sandra E. Esparza" <[84]SEsparza <@t> seton.org>,
   =9[85]histonet <@t> lists.utsouthwestern.edu
   Message-ID:          <601376475.17568918.1400601[86]556617.JavaMail.ro=@neo.tamu.edu>
   Content-Type: text/plain; charset=utf-8
   Thanks Sandra! My next step was to try that.
   Dakshna
   ----- Original Message -----
   From: "Sandra E. Esparza"=[87]SEsparza <@t> seton.org>
   To: "Dakshnapriya Balasubbram=ian" <[88]dakshnapriya <@t> neo.tamu.edu>
   Sent: Tues�y, May 20, 2014 10:34:25 AM
   Subject: RE: [Histonet] HELP- Cryosectio=ng FAT!
   You  might  want  to  dry  Liquid  Nitrogen on the face of t= block
   before you cut it.
   Sandra
   Sandra Esparza H=ASCP), QIHC
   Histotechnologist Mohs
   Austin Dermatologic Surgery�nter
   512-324-7468 x84027
   [89]sesparza <@t> seton.org
   -----Original Message-----
   From:   [90]his=net-bounces <@t> lists.utsouthwestern.edu  [[91]mailto   :histonet-bounces <@t> lists.utsouthwestern.edu]      On      Behalf     Of
   Balasubbramani=, Dakshnapriya
   Sent: Tuesday, May 20, 2014 10:23 AM
   To: [92]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] =LP- Cryosectioning FAT!
   Hi all,
   I've  been  having  pr=lems with cryosectioning fatty tissue for a
   long  time now. The section le=es a hole in the middle. I know this
   is  probably  a  much  discussed  topic,  =t  I've  tried a lot of
   strategies  with  no  luck.  The  tissue  is of mouse ori=n and has
   micro-lesions buried inside fat. So the fat needs to be cut in =der
   to get to the lesion. I started with a temperature of -17C and tried    upto  -25C and also at -50C; didn't work at any of these temperatures.
   I  tri�  rubbing  the  block,  blade  and anti-roll plate with dry
   ice-again,no luck.   I  rapid-freeze  the tissue with OCT in LN2 and then store the b=ck
   at  -80C.  Could I try sectioning the block directly from -80C? (never
   d=e that).
   Any other suggestions on how to tackle this?
   Any advice is much appreciated!!
   Thanks,
   Dakshna
   <= />_______________________________________________
   Histonet mailing =st
   [93]Histonet <@t> lists.utsouthwestern.edu
   [94]http://lists.utsouthwestern.edu/mailman/listi=o/histonet
   CONFIDENTIALITY NOTICE:
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   Message: 11
   Date: Tue, 20 May 201A1:03:08 -0500 (CDT)
   From: "Balasubbramanian, Dakshnapriya"=
   <[95]dakshnapriya <@t> neo.tamu.edu>
   Subject: Re: [Histon=] HELP- Cryosectioning FAT!
   To: Laurie J King <[96]k=g.laurie <@t> marshfieldclinic.org>,
        histonet=ists.utsouthwestern.edu
   Message-ID:
        <262036458.175957e.1400601[97]788819.JavaMail.root <@t> neo.tamu.edu>
   =ntent-Type: text/plain; charset=utf-8
   Hi Laurie,
   =,  the  tissue hasn't been fixed before freezing. My prof doesn't
   prefer  th=ethod,  but if nothing else works, we might give it a
   try.
   An=uggested protocols for fixation?
   Thanks!
   Dakshna
   ----- Original Message -----
   From:     "Laurie     J     King"    <<�lass="parsedEmail"
   href="mailto:king.laurie <@t> marshfieldclinic.org"                   ta   rget="_blank">king.laurie <@t> marshfieldclinic.org>
   To: "Dak=napriya Balasubbramanian" <[98]dakshnapriya <@t> neo.tamu.edu>
   Sent: Tuesday, May 20, 2014 10:37:35 AM
   Subject: RE: [Histo=t] HELP- Cryosectioning FAT!
   Dakshna,
   Has this tiss= been fixed before freezing by any chance?
   laurie
   -=--Original Message-----
   From:          [99]histonet-bou=es <@t> lists.utsouthwestern.edu
   [[100]mailto:histonet=ounces <@t> lists.utsouthwestern.edu] On Behalf Of
   Balasubbramanian, Daksh=priya
   Sent: Tuesday, May 20, 2014 10:23 AM
   To: [101]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] HELP- Cryo=ctioning FAT!
   Hi all,
   I've  been  having  problems  wit=ryosectioning fatty tissue for a
   long  time now. The section leaves a hol=n the middle. I know this
   is  probably  a  much  discussed  topic,  but  I've  t=ed a lot of
   strategies  with  no  luck.  The  tissue  is of mouse origin and ha=
   micro-lesions  buried  inside fat. So the fat needs to be cut in order
   to g= to the lesion. I started with a temperature of -17C and tried
   upto   -25C  =d  also  at  -50C;  didn't  work  at  any  of  these
   temperatures.  I  tried  rubbing=e block, blade and anti-roll plate
   with dry ice-again,no luck.
   I  rapid-freeze the tissue with OCT in LN2 and then store the block at
   -8
.  Could  I try sectioning the block directly from -80C? (never
   done that).   Any other suggestions on how to tackle this?
   Any ad=ce is much appreciated!!
   Thanks,
   Dakshna
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   End of Histonet Digest, Vol 126, Issue 20
   ***********=****************************


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  46. file://localhost/tmp/3D"h  47. 3D"mailto:CThornton <@t> dahlchase.com"
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  51. 3D"mailto:cthornton <@t> dahlchase.com"
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  65. ="mailto:Histonet <@t> lists.utsouthwestern.edu"
  66. 3D"http://list=
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  69. 3D"mailto:407431.JavaMai  70. 3D"mailto:azdudley <@t> hotmail.com"
  71. 3D"mailto:Histonet <@t> li  72. 3D"mailto:histonet <@t> lis  73. ="mailto:279A33E8D42CCF0D13D0 <@t> phx.gbl"
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