[Histonet] HTL

madary <@t> verizon.net madary <@t> verizon.net
Wed May 21 05:54:10 CDT 2014

   &nb   cover  to cover   to cover.

   Nick(Rocky) Madar   Joni Madary, PhD(in life)

   On 05/20/14, hist

   Send Histonet    	[1]histonet <@t> lists.utsout   To subscribe or unsubscribe via the World Wide    	[2]http://lists.utsouthwe   or, via email, send a message    	[3]histonet-r   You can reach the person man   	[4]histonet-owner <@t> lists.utso   When replying, please edit your Subject line    than "Re: Contents of Histonet digest...&q   Today's Topics:
   1. HTL certification (C   2. RE: HTL certification (Esther C Peters)
   3. pos   (Pathology-Histology S   4.  immunohistochemisty  coding (Michael LaFriniere) 5. antibody vials
   (Clare Thornton)
   6. RE: antibody vials (Mu   7. HELP- Cryosectioning FAT! (Balasubbramanian, Daks   8. new processor (anita)
   9. RE: antibody vials (A   10. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Da   11. Re: HELP- Cryosectioning FAT! (Balasubbramanian, Dak   -----------------------------------------------   -----------------------
   Message: 1
   Date: Mon, 19 May 2014 1   From: Cecy Stan <[5]cecystan76 <@t> gmail.com>
   Subject: [Histonet] HTL certification
   To: [6]histone   Message-ID:
   	<CACh=0wgaaEfV   DFmsuB-Jw8OKo=LKUP[7]ApGujjkoEAgjAzASxo9g <@t> mail.gmail.com   Content-Type: text/plain; charset=UTF-8
   Hello ev   I'm starting to prepare for my HTL certification (I am v   and anxious but also very excited about this decision to g   I  was  just  curious  to know how you guys prepared for    long it
   took  for  you  to  prepare  before  taking  the test. Wi   preparation
   be enough? (I know that may depend on the indi   I
   had  my  Masters  over  10 years ago and I haven'   since
   I  have  Freida  L.  Carson's     memorize --
   but  the  outline ASCP   you
   have any other bo   Thank you in advance for your input an   C.A.
   Message: 2
   Date: Mon, 19 May 2014 17:46:47 +0000
   From: Esther   Subject: RE: [Histonet] HTL ce   To: Cecy Stan <[9]cecystan76 <@t> gmail.com>,
      	<[11]histonet <@t> lists.utsouthwestern.edu>
   Me   Content-   Hi C.A.,
   I  don't have the HT or HTL, but from my college teaching experience    in  histology  and  histotechniques, I just wanted to caution you that
   memoriz   concepts, so th   able to think through   the situation. I see this    things  they  memorize,  but then t   can  figure  things  out.  One  of  the new tea   students  prepare  "concept maps," to see the   and  terms,  and  these  linkages  will help you in the   histology examples, see:
   [13]http://www.biologycorner.com/anatomy/tissues/tissue_conce   pt_map_samples.html
   I don't know of any concept maps for his   I am going to add this to my course next year!   Esther
   Esther C. Peters, Ph.D.
   Assistant Profes   Environmental Science & Policy
   George Mason University
   4400 University Drive, MS 5F2
   Fairfax, VA 22030-4444
   Office: D   Phone: 703-993-3462
   Fax: 703-993-1066e-mail: [14]epeters2 <@t> gmu.edu
   [15]https://bluprd0511.outlook.com/owa/redir.aspx?C   =ET8XhF-xC0ytBErXdaN3U3lGqWmZNdAI_N-4nsEb0IjgUpeIoQa7EcVMJMh2oePPPKr
   rDjhw   ______________   From: [16]histonet-bo   ounces <@t> lists.utsouthwestern.edu>  on  behalf  of  Cecy  Stan <[18]cec   ystan76 <@t> gmail.com>
   Sent: Monday, May 19, 2014 1:32 PM
   To:    Subject: [Hist   Hello everyone,
   I'm starting    and anxious but   I was just   it
   took  fo   preparation
   be    I
   ha   since
   t   I  have  Freida  L.  Carson's  3rd Edition book -- quite daunti   memorize --
   but  the outline ASCP has provided for study seems t   you
   have any other book/study aid suggestions?
   <   C.A.
   _______   Histonet mailing list
   [20]Histonet <@t> lists.utsouthwestern.edu
   [21]http://lists.utsouthwestern.edu/mailman/listinfo/histonet   ------------------------------
   Messa   Date: Tue, 20 May 2014 12:09:28 +0000
   From: "Pathology   Subject:   To: "[23]histonet <@t> lists.utsouthwestern.edu"
   	<[24]histonet <@t> lists.utsouthwestern.edu>
   Cc: "[25]tahseen0009 <@t> gmail.com" <[26]tahseen0009 <@t> gmail.com>Message-ID:
   	<BBFD4ECF5CBFAE4E936663D   Content-Type: text/plain;   Hi All,
   We  are  going  to  opt   expression   in  breast  cancer  tissue   formalin fixed paraffin sections.
   I  shall     "positive control   Regards
   Muhammad Tahseen
   Sr.Supervisor Histopathology
   SKMCH&RC Lahore
   -----------------------   Message: 4
   Date: Tue, 20 May 2014 13:03:55 +0000
   From: Michael LaFriniere <[27]Michael.LaFriniere <@t> ccplab.com   Subject: [Histonet] immunohistochemisty coding
   To:     "   @stdavids.com>,
   	"[30]histonet <@t> lists.utsou   	<[31]histonet <@t> lists.utsout   Message-ID:
   	<4A2A16B9707CE04E9C[32]B6C82DC18C1D29670A90 <@t> AHCMSASEXCH02.my.ahc.lo
   Content-Type: text/plain; charset="us-ascii"
   Hi Sarah,
   Joyce  is  correct,  under medicare changes in patho   can  not  bill  for  each  of  the  blocks  of  the  same  antibody a   pplications,  only  one  charge per specific antibody mentioned in the
   patholo   "specimen"   s100  for  the sam   blocks,  under  medicare  y   when the need is appropriate,
   I  must  voice  my opinio)- this probably will lower some of the over   utilization  and  inappropriate billing practices that many of us have
   seen     capitalized   on   the   reimbursements  and  change in medicare   who have used the utilization of immunohisto   However,  "under  current  2014&   you bill for the additional work per b   cpt  code,  I am sure moving forward we are go   adjustments to the pathology coding processes!
      Michael R. LaFriniere, HT (ASCP)
   Executive Director
   Capital Choice Pathology Laboratory
   12041 Bournefield Way, Suit   P: 240.471.3427 * F: 240.471.3401 * Ce   [33]michael.lafriniere <@t> CCPLab.com
      -----Original Message-----
   From:        [34]histonet-bounces <@t> lists.utsouthwestern.edu        [ma   ilto:histonet-bounces <@t> lists.utsouthwestern.edu]  On  Behalf  Of Weems,
   Joy   Sent: Monday, May 19, 2014 12:21 PM
   To: '[35]Sarah.Dy   [36]histonet <@t> lists.utsouthwestern.edu   Subject: [Histonet] RE: Billing
   No - one G0461.
   <   and G0462   Joyce Weems
   Pathology Manager
   678-843-7376 Pho   678-843-7831 Fax
   [37]joyce.weems <@t> emoryhealthcare.o   [38]www.saintjosephsatlanta.org
   5665 Peachtree Dunwoody Road
   Atlanta, GA 30342
   This  e-ma   Joseph's  Hospital  an   recipient(s).  It  may  contai   confidential.   Any   unauthorized   review   distribution  is  prohibited. If you are not the intend   please  delete  this  message,  and reply to the sender regarding   error in a separate email.
   -----Original Message-----
      [[40]mailto:histonet-bounces <@t> lists.utsouthwestern.e   [41]Sarah.Dysart <@t> stdavids.com
   Sent: Mon   To: [42]histonet <@t> lists.utsout   Subject: [Histonet] Billing
   When it comes    So you have one specimen tha   AE1/AE3 is ordered on all 10 blocks.
   Can  you  bil   Additional (G0462) nine t   Thanks,
   Sarah E. Dysart, BA, HT (ASCP), QIHC (A   David's North Austin Medical Center
   1222   Austin, Texas 78758
   Histonet mailing   [43]Histonet <@t> lists.utsouthwestern.edu
   [44]http://lists.utsouthwestern.edu/mailman/list   ________________________________
   Th   of
   t   privileged
   i   recipien   distribution
   or cop   prohibite   If you have received this message in error, please contact
   the sender by reply e-mail message and destroy all copies of the
   or   ________________________   Histonet mailing list
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   <   ------------------------------
   Message: 5
   Date:    From: Clare Thornton <[47]CThor   Subject: [Histonet] antibody vials
   To:      Mes   	<C9D78FFC9D668B4CBEA4405F[50]84697   504013D73DEB4F1 <@t> iris.dahlchase.net>
   Content-Type: text/plain; c   Does  anyone  have  any  good  ideas fo   concentrated  antibodies  neat and organized in   we  keep  ours  in empty pipette tip boxes (without ti   they're  always  falling over and mixed up. We use several di   vendors  for  our concentrated antibodies, so our vials are all diffe   rent sizes. Thanks for any ideas!
   Clare J. Thornton   Lead Immunohistochemistry Technologist
   Dahl-Chase   417 State Street, Suite 540
   Bangor, ME 04401   [51]cthornton <@t> dahlchase.com<[52]mailto:cthornton <@t> dahl   ----------------------------   Message: 6
   Date: Tue, 20 May 2014 14:59:21 +0000
   Fr      To: Clare Thornton <[54]   	"histonet <@t> l   	<[55]histonet <@t> li   Message-ID:
   	<A0CD799E6FCD494   armanos.org>
   Content-Type: text/plain; charset="us-ascii   I  use  cardboard  cryovial  boxes and they work quite well.    remove some of the dividers to make space for the larger vials.
   eze   Valerie    Research Assistant
   Department   Karmanos Cancer Institute
   4100 John R
   Detroit, MI 48201
   Telephone: (313) 576 8282   Fax: (313) 576 8306
   E-mail: [58]murphyv <@t> karmanos.or   Better treatments. Better outcomes.
   From:                    [59]histonet-bounces <@t> lists.utsouthwestern.edu
   [[60]histonet-bounces <@t> lists.utsouthwestern.edu]  on  behalf  of  Clare
   Thornto   Sent: Tuesday, May 20, 2014 1   To: [62]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] antibody vials
   Does  anyone  have  any  good     concentrated  antibodies neat and orga   we  keep  ours  in empty pipette tip boxes (wi   they're  always  falling over and mixed up. We use se   vendors  for  our  concentrated  antibodies,  so  our  vials are a   different sizes. Thanks for any ideas!
   Clare J.    Lead Immunohistochemistry Technologist
   Da   417 State Street, Suite 540
   Bangor,    [63]cthornton <@t> dahlchase.com<[64]mailto:cthornto   __________________________________   Histonet mailing list
   [65]Histonet <@t> li   [66]http://li   -----------   Confidentiality Notice: This email message, including any attachments   ,  is  for  the  sole use of the intended recipient(s) and may contain
   confiden   intended   recipient(s   dissemination,  unauthorized review, use   of  this  email  and  any  materials contained in a   prohibited.  If  you  receive  this message in error, or are    intended recipient(s), please immediately notify the sender by emai   and destroy all copies of the original message, including attachments.
   Message: 7Date: Tue, 20 May 2014 10:23:27 -0500 (CDT)
   From: "Balasubbra   <[67]dakshnapriya <@t> neo.tamu.edu><   Cryosectioning FAT!
   To: [68]histonet <@t> lists.utsouthwestern.edu
   	<21128   Content-Type: text/plain; charset=utf-8
   Hi all,
   I've  been  having problems with cryosectioning fatty tissue for a lo   ng  time  now. The section leaves a hole in the middle. I know this is
   probab   with  no  luck.   buried inside fat. So    lesion. I started with a tem   also  at  -50C;  didn't  work  at any o   rubbing  the  block, blade and anti-roll plate    luck.
   I  rapid-freeze  the tissue with OCT    at -80C. Could I try sectioning the block d   done that).
   Any other suggestions on    Any advice is much appreciated!!
   ------------------------------<   Message: 8
   Date: Tue, 20 May 2014 10:26:13 -0500
   From:   Subject: [Histonet] new p   To: "[71]Histonet <@t> lists.utsouthwestern.edu   	<[72]histonet <@t> lists.utsouthwestern.edu<   Message-ID: <COL131-W4520B92[73]279A33E8D42CCF0D   Content-Type: text/plain; charset="iso-885   Thanks  everyone  for  your input on processors, I think   with the VIP, it has been a good machine just getting older n   everyone have a great day!!!!
   Anita Dudley
   Providence Hosp
   Mobile, Alabama 3   		 	 		
   ------------------------------<   Message: 9
   Date: Tue, 20 May 2014 08:29:05 -0700
   From:   Subject: RE: [Histonet] antibody vials
   To: "Clare Thornton"   	<[76]histonet <@t> lists.utsouthwestern.edu>
   	<4A   Content-Type: text/plain;	charset="us-ascii"   We got some plexiglass dividers at the "Container Store&q   have one
   near  or check online. Can't remember what they w   had
   different  sized  square  compartments  that  fit  v   antibodies.
   -----Original Message-----<   [78]histonet-bounces <@t> lists.utsouthwestern.   [[79]mailto:histonet-bounces <@t> lists.utsout   Clare
   Sent: Tuesday, May    To: [80]histonet <@t> lists.utsouthwestern.e   Subject: [Histonet] antibody vials
   Does anyone have    concentrated  antibodies   we
   keep  ours  in  empty  pi   they're
   always  falling  over    for
   our concentrated a   Thanks for any ideas!   Clare J. Thornton, HTL(ASCP)QIHC
   Lead Immunohi   Dahl-Chase Diagnostic Services
   417 Stat   Bangor, ME 04401
   cthornton <@t> dahlchase.   _   Histonet mailing list[82]Histonet <@t> lists.utsouthwestern.edu
   [83]http://lists.utsouthwestern.edu/mailman/listinfo/   ------------------------------
   Message: 10
   Date: Tue, 20 May 2014 10:59:16 -0500 (CDT)
   From:    Subject: Re: [Histonet] HELP- Cryosectioning FAT!
   To: "Sandra E. Esparza" <[84]SEsparza <@t> seton.org>,
      Message-ID:   	<601376475.17568918.1400601[86]556617.JavaMail.ro   Content-Type: text/plain; charset=utf-8
   Thanks Sandra! My next step was to try that.
   ----- Original Message -----
   From: "Sandra E. Esparza"   To: "Dakshnapriya Balasubbram   Sent: Tues   Subject: RE: [Histonet] HELP- Cryosectio   You  might  want  to  dry  Liquid  Nitrogen on the face of t   before you cut it.
   Sandra Esparza H   Histotechnologist Mohs
   Austin Dermatologic Surgery   512-324-7468 x84027
   [89]sesparza <@t> seton.org
   -----Original Message-----
   From:   [90]his   :histonet-bounces <@t> lists.utsouthwestern.edu]      On      Behalf     Of
   Balasubbramani   Sent: Tuesday, May 20, 2014 10:23 AM
   To: [92]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet]    Hi all,
   I've  been  having  pr   long  time now. The section le   is  probably  a  much  discussed  topic,     strategies  with  no  luck.  The  tissue  is of mouse ori   micro-lesions buried inside fat. So the fat needs to be cut in    to get to the lesion. I started with a temperature of -17C and tried    upto  -25C and also at -50C; didn't work at any of these temperatures.
   I  tri   ice-again,no luck.   I  rapid-freeze  the tissue with OCT in LN2 and then store the b   at  -80C.  Could I try sectioning the block directly from -80C? (never
   d   Any other suggestions on how to tackle this?
   Any advice is much appreciated!!
   <   Histonet mailing    [93]Histonet <@t> lists.utsouthwestern.edu
   [94]http://lists.utsouthwestern.edu/mailman/listi   CONFIDENTIALITY NOTICE:
   This   email   m   confidential  and  may  contain p   for   the   named   recipient(s).  If  you  are     recipient(s),  you  are  hereby  notified  that  the  disseminati   distribution,  and  or copying of this message is strictly prohibited.
   If   recipient(s),  plea   delete  this email from you   form   immediately.   Receipt   by  anyo   recipient(s)  is  not a waiver of any attorney-client   or other applicable privilege.
   -----   Message: 11
   Date: Tue, 20 May 201   From: "Balasubbramanian, Dakshnapriya"   <[95]dakshnapriya <@t> neo.tamu.edu>
   Subject: Re: [Histon   To: Laurie J King <[96]k   	histonet   Message-ID:
   	<262036458.175957      Hi Laurie,
      prefer  th   try.
   An   Thanks!
   ----- Original Message -----
   From:     "Laurie     J     King"    <<   href="mailto:king.laurie <@t> marshfieldclinic.org"                   ta   rget="_blank">king.laurie <@t> marshfieldclinic.org>
   To: "Dak   Sent: Tuesday, May 20, 2014 10:37:35 AM
   Subject: RE: [Histo   Dakshna,
   Has this tiss   laurie
   -   From:          [99]histonet-bou   [[100]mailto:histonet   Balasubbramanian, Daksh   Sent: Tuesday, May 20, 2014 10:23 AM
   To: [101]histonet <@t> lists.utsouthwestern.edu
   Subject: [Histonet] HELP- Cryo   Hi all,
   I've  been  having  problems  wit   long  time now. The section leaves a hol   is  probably  a  much  discussed  topic,  but  I've  t   strategies  with  no  luck.  The  tissue  is of mouse origin and ha   micro-lesions  buried  inside fat. So the fat needs to be cut in order
   to g   upto   -25C     temperatures.  I  tried  rubbing   with dry ice-again,no luck.
   I  rapid-freeze the tissue with OCT in LN2 and then store the block at
   -8   done that).   Any other suggestions on how to tackle this?
   Any ad   Thanks,
   _____   Histonet mailing list
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   The  contents  of this message may contain private, protec   privileged  information.  If you received this message in error,    should  destroy  the e-mail message and any attachments or copies, and
   yo   any  info   advise  of  the  erro   Thank you for your cooperati   ------------------------------
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