[Histonet] RE: anyone out there doing a DMAB-nitrite stain for tryptophan?

[Wolfe, Christina]

As a follow up to my original questions - I now understand the ceolloidinise is the procedure using celloidin.  I am still having trouble finding a vendor for celloidin.  Can I substitute LVN in place of the celloidin?  Or maybe there is another method to use in this step.  Any feedback will be greatly appreciated!

Kristie

Christina Wolfe, BS, MLT (ASCP), HT, QIHC
Drug Safety Evaluation/Bristol-Myers Squibb
Pathology Dept.
812-307-2093


From: Wolfe, Christina
Sent: Monday, May 12, 2014 2:25 PM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: anyone out there doing a DMAB-nitrite stain for tryptophan?

Hi,
We are wanting to do the DMAB-nitrite method for tryptophan (Bancroft is the reference).  I have some questions about the method.  If anyone has experience, please contact me.  Below are my questions:


1.        Take sections to alcohol.  (What percentage?  Not sure if this matters).

2.       Celloidinise in 0.5 per cent celloidin.    (Where can I find celloidinise - is this still sold commercially?)

3.       In step 6 of the procedure there is an acid alcohol rinse (anyone know what the acid alcohol concentration should be?)

Thanks in advance if you can help!

Kristie

Christina Wolfe, BS, MLT (ASCP), HT, QIHC
Drug Safety Evaluation/Bristol-Myers Squibb
Pathology Dept.
812-307-2093



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