[Histonet] RE: IHC on animals

Wed Mar 26 16:55:31 CDT 2014

Lester,

Probably not in most cases but it depends upon what the antibody is, whether
as a an antihuman ab it also cross reacts to the species you are interested
in and what detection reagents you are using.
For instance if you have determined a rabbit anti human antibody also cross
reacts in mouse tissue, you could use a detection such as for the Leica Bond
by leaving out the link (they call it the post primary but it is rabbit anti
mouse IgG to link mouse antibodies to the goat anti rabbit labeled polymer
second step), since your ab is rabbit it would link directly to the anti rab
labeled polymer and the anti mouse link which would bind non specifically to
the mouse endogenous Ig can be avoided. 
You would have to do a lot of research to first determine if the anti human
rabbit antibody you want to use (it should not be made in a mouse) cross
reacts to mouse tissue.  The species you are wanting to label is also very
important, this system would only work for anti human rab abs that also
react in mouse, rat or any other species except rabbit and goat because
those are the species the detection is made in or against.
We have research versions of the Leica Bond instruments which allows us to
manipulate the instrument and detection for these purposes including
replacing the rab anti ms link with another link, I am not sure if the pure
clinical versions of the Bond allow this.
Cheers,
Patsy

Patsy Ruegg, HT(ASCP)QIHC
Ruegg IHC Consulting
40864 E. Arkansas Ave
Bennett, CO 80102
H 303-644-4538
C 720-281-5406
pruegghm <@t> hotmail.com 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Elizabeth
Chlipala
Sent: Wednesday, March 26, 2014 2:20 PM
To: Lester Raff MD; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: IHC on animals

Lester

I would not, first of all not all antibodies that work in human will cross
react with a particular species of animal.  Another thing to consider is
that most human based detection systems consist of dual link reagents,
meaning that they work on both mouse and rabbit primaries these detection
system are not ideal for working with animals and depending upon the species
that you are working with may cause some problems in with background
staining due to the secondary antibody or polymer binding to endogenous IgG
in the samples.  You can initially pilot your protocol on a particular
species to see what happens but I would not take the chance and run a bunch
of slides thinking that the protocol that you have in place will work in the
particular species of animal you need to stain.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
18592 Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

March 10, 2014 is Histotechnology Professionals Day

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lester Raff
MD
Sent: Wednesday, March 26, 2014 2:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on animals

Hi:

Can IHC validated for humans be performed on animal tissues without any
protocol changes?

Thanks,

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet