[Histonet] RE: IHC on animals

[Elizabeth Chlipala]

Lester

I would not, first of all not all antibodies that work in human will cross react with a particular species of animal.  Another thing to consider is that most human based detection systems consist of dual link reagents, meaning that they work on both mouse and rabbit primaries these detection system are not ideal for working with animals and depending upon the species that you are working with may cause some problems in with background staining due to the secondary antibody or polymer binding to endogenous IgG in the samples.  You can initially pilot your protocol on a particular species to see what happens but I would not take the chance and run a bunch of slides thinking that the protocol that you have in place will work in the particular species of animal you need to stain.

Good Luck

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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liz <@t> premierlab.com
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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Lester Raff MD
Sent: Wednesday, March 26, 2014 2:03 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] IHC on animals

Hi:

 

Can IHC validated for humans be performed on animal tissues without any protocol changes?

 

Thanks,

 

Lester J. Raff, MD MBA

UroPartners

Medical Director Of Laboratory

2225 Enterprise Dr. Suite 2511

Westchester, Il 60154

Tel: 708-486-0076

Fax: 708-492-0203

 

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