[Histonet] RE: Paraffin Effects on IHC

[Elizabeth Chlipala]

Phil

Are the slides prepared from the different paraffins being testing run on the same H&E stain run or on different runs?

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Manfre, Philip
Sent: Tuesday, March 25, 2014 12:28 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Paraffin Effects on IHC

Our lab is considering switching paraffin in our histology labs.  They are being evaluated with an emphasis on routine H&E staining.  The IHC lab has some concerns about the effects this may have on IHC staining.  We have noticed slight differences in staining intensity already, during some evaluation experiments.  Does anyone have opinions either way, positive or negative, on the following products?  Of particular emphasis are any effects on the staining intensity (which also could relate to the ease of deparaffinization of the sections), ability to stain evenly, or anything else you may feel is relevant.


1.       Fisher Scientific TissuePrep T-565

2.      Leica Paraplast

3.      ThermoScientific Histoplast

Thanks all for your expertise! It's great that we have such a global forum for our field.

Phil.

Philip Manfre, B.A., HT (ASCP)
Associate Principal Scientist
Merck Research Laboratories
WP45-251
PO Box 4
West Point, PA 19486

215-652-9750
215-993-0383 (fax)
philip_manfre <@t> merck.com<mailto:philip_manfre <@t> merck.com>


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