[Histonet] IHC antibody optimizing & validating

[Davis, Cassie]

Will you help me? I understand we are to use the known positives controls that the manufactures' recommends in the package insert when optimizing the stains, but I need to know what is your general procedure for optimizing (how many different staining protocols do you test) and validating a new antibody (how many different or "known" positive and negative tissues do you test [predictive markers I understand are 20])?

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095



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