[Histonet] RE: IHC Validation

[Cartun, Richard]

Are you changing the antibody dilution?  If so, I would run 2-3 positive and negative cases and make sure that the immunoreactivity compares favorably to what you observed before.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Tuesday, March 11, 2014 5:27 PM
To: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] IHC Validation

If I have validated an antibody at a specific incubation time, and then later want to decrease the incubation time, do I have to re-validate??

Laurie Colbert, HT (ASCP)
Histology Supervisor
PATH MD
8158 Beverly Blvd.
Los Angeles, CA  90048
(323) 648-3214 direct
(424) 245-7284 main lab

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