[Histonet] RE: CAP Annual Results Comparison for FISH/ISH

[Cartun, Richard]

I'm not aware of published benchmarks for FISH/ISH, but if you're doing IHC for ER, PR, and HER2 in breast CA you may find the following information useful:

Lal P, et al:  ER and PR and histologic features in 3,655 invasive breast carcinomas.  Am J Clin Pathol 2005;123:541-546.

ER+ tumors - 74%
PR+ tumors - 49
HER2+ tumors - 16%


Fitzgibbons PL, et al:  Recommendations for validating ER and PR IHC assays.  Arch Pathol Lab Med 2010;134:930-935.

For women over 65 years of age, the % of negative cases should not exceed 20%.
For "low-grade" invasive carcinomas, the proportion of negative cases should not exceed 5%.


My own data for invasive breast CA:

ER+ tumors - 85%
PR+ tumors - 70%
HER2+ tumors - 14%


Please keep in mind that with the introduction of new monoclonal antibodies, more sensitive detection systems, and the "recommendation" that tumors with "1%" immunoreactive cells be called "Positive", the "old" benchmarks for ER and PR are no longer valid.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org




-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of O'neil, Beth
Sent: Wednesday, March 12, 2014 2:41 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH

Would fellow Histonetters be able to explain how they answer the following CAP question:
ANP.22970 For immunohistochemical and FISH/ISH tests that provide independent predictive information, the laboratory at least annually compares its patient results with published benchmarks, and evaluates interobserver variability among the pathologists in the laboratory.
Where would one even find published benchmarks?  Thank you

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor/Technical Specialist West Virginia University Hospitals oneilb <@t> wvuhealthcare.com
304-293-7629 (office)
304-293-6014 (lab)




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