[Histonet] RE: MUM-1

joelle weaver joelleweaver <@t> hotmail.com
Thu Mar 13 13:36:42 CDT 2014


I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it stains great on tonsil and kidney tubules.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: LSebree <@t> uwhealth.org
> To: CDavis <@t> che-east.org; histonet <@t> lists.utsouthwestern.edu
> Date: Thu, 13 Mar 2014 16:19:11 +0000
> CC: 
> Subject: [Histonet] RE: MUM-1
> 
> This is our protocol for the Ultra; maybe it will help.
> 
> 64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4".  This is with UltraView DAB detection.  We use tonsil as well however we validated with HD, LN, GI, tonsil, etc.
> 
> Good luck!
> 
> Linda A. Sebree 
> University of Wisconsin Hospital & Clinics 
> IHC/ISH Laboratory 
> 600 Highland Ave. 
> Madison, WI 53792 
> (608)265-6596 
> FAX: (608)262-7174 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
> Sent: Thursday, March 13, 2014 8:54 AM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] MUM-1
> 
> Good morning Histonet Folks,
> 
>          I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this.
> 
> Cassandra Davis
> CDavis <@t> che-east.org
> 302-575-8095
> 
> 
> 
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