[Histonet] RE: MUM-1

[Sebree Linda A]

This is our protocol for the Ultra; maybe it will help.

64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4".  This is with UltraView DAB detection.  We use tonsil as well however we validated with HD, LN, GI, tonsil, etc.

Good luck!

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
Sent: Thursday, March 13, 2014 8:54 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] MUM-1

Good morning Histonet Folks,

         I am hoping one of you will help me. I am in the process of optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark XTstainer and I am not thrilled with the results I am getting. I have tried the "usual adjustments" and the results are less than optimal in my opinion. I am using a normal tonsil control right now but if you have another suggestion please do not hesitate to recommend. I am praying somebody might have done this before and would be willing to share their staining protocol or tips with this.

Cassandra Davis
CDavis <@t> che-east.org
302-575-8095



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