[Histonet] RE: Cornflaking artifact

Thu Mar 13 10:59:06 CDT 2014

We had this problem several years ago.  We  were using the sakura tapes 
with the coverslipper. 
We did the following: 

Last three alcohols were changes frequently. 
Slides should be not dry when loading on coverslipper.
If you could load two racks at a time, only load one.  By this way the 
slides in the second rack will not dry out. 
Finally, change the tapes from sakura to Mercedes Medical tapes.

Mala

Nirmala Srishan
Holy Name Medical Center

From:   "HERRINGTON, SHEILA" <SHEILA.HERRINGTON <@t> interiorhealth.ca>
To:     'Laurie Colbert' <lcolbert <@t> pathmdlabs.com>, Sharon Scalise 
<SSCALISE <@t> beaumont.edu>, "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>
Date:   03/13/2014 11:43 AM
Subject:        [Histonet] RE: Cornflaking artifact
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu

We also have recently started to see this artifact more than ever before, 
and nothing in our process has changed.  We have tried everything to 
correct to no avail.  Wonder if it is possible to be a change in some type 
of supply, either xylene or coverslipping film.  Something has changed but 
am at a loss as to what.

Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
Sheila.herrington <@t> interiorhealth.ca

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Laurie 
Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

You will also see the cornflaking if your tissue is lifting off of the 
slide at all.  We used to get this more often on hard, decal specimens 
than on other specimens.  We used the film to coverslip.  If you remove 
the film from the problem slides and recoverslip conventionally with extra 
mountant and glass coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon 
Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with "cornflaking" (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper 
to make sure it was functioning properly, including the xylene drip.  We 
continue to have this artifact and it is driving us crazy.  It is sporadic 
with no pattern of tissue type or placement on the slide.  Sometimes it 
lands on tissue other times not.  Most of the time when we remove the 
coverslip and re-coverslip it goes away (I am assuming because the acetone 
removes any minute amounts of water that may be present).  We just cannot 
figure out where the water is coming from.  Has anyone seen this artifact 
while using the drying step on the prisma stainer?  We just recently 
started using the drying on some slides and I am thinking maybe it is 
causing humidity???  I cannot say for a fact that our "cornflaking" 
started at the same time, but it is suspicious. HELP!!!!!

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [
mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Wait, 
Trevor Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffin....what 
is the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double 
tetracycline label?

Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S. 
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