[Histonet] RE: ISH Automation

[Terri Braud]

Hi - there are a couple of instruments that automate ISH and RISH.  Our
lab was a beta site for BioCare's Oncore and we loved it.  It has an
open platform and is very user friendly. The Oncore is a very compact
instrument and the maintenance was easy and minimal. All of the techs
here liked it.
Check it out.

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 6
Date: Wed, 25 Jun 2014 08:38:29 -0400
From: Emily Brown <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] ISH preparation
To: Jennifer.Johnson <@t> genzyme.com

That may be what you have to do, as the first day of ISH needs to be
RNase free!
When I do them, it is 10 slides at a time and we have dishes and a lab
bench set aside for RNase free procedures only.  Obviously this is not
feasible in all workplaces!
I would just think about it logically.  Use gloves when you can, always.
 Can you keep the hybridizing part RNase free? I don't know what
machines you use for this--we do process the slides manually in glass
dishes and then hybridize in a slide container and oven kept for RNase
free purposes.
 After hybridization, you don't really need to be too careful.

Now I'm wondering if there's some sort of magical machine that does ISH!

Emily

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