[Histonet] Fwd: Histonet Digest, Vol 127, Issue 25 - Control slides

Barbara Foster bfoster <@t> mme1.com
Fri Jun 20 15:06:48 CDT 2014


There is new technology available now  for standardizing color images 
so, especially if you are taking images of control slides of stained 
sections, consider using it.  On the acquisition side, it 
standardizes the system and camera response using a calibration slide 
with a matrix of known colors.  The software also auto adjusts 
brightness and white balance, resulting in highly consistent, 
reproducible results.  On the display side, the kit has an easy to 
use colorimeter which provides empirical calibration of the monitor 
(not just an electronic look up table).  Called ChromaCal 
(chromacal.com), it will be on display this next week at Soc. for Tox 
Path (STP, Washington DC).

Hope this is helpful,
Barbara Foster

Caveat: No commerical interest


>Delivered-To: bfoster <@t> 1535597.1652944
>Date: Thu, 19 Jun 2014 13:02:40 -0400
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>Subject: Histonet Digest, Vol 127, Issue 25
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>Today's Topics:
>
>    1. RE: Balance and Scale Calibration (Elizabeth Chlipala)
>    2. Full Time Day Shift HT/HTL Position in Sioux Falls,       South
>       Dakota (Matthew Lauterbach)
>    3. RE: control slides (Mayer,Toysha N)
>    4. Liver bxs (Amanda Coscetti)
>    5. RE: VIP 1000 (Tony Reilly)
>    6. RE: Liver bxs (Mike Pence)
>    7. Anti-PLA2r (Breckenridge, Richard A)
>    8. Re:  VIP 1000 charcoal filters (gayle callis)
>    9. Re: Liver bxs (Rena Fail)
>
>
>----------------------------------------------------------------------
>
>Message: 1
>Date: Wed, 18 Jun 2014 11:02:52 -0600
>From: Elizabeth Chlipala <liz <@t> premierlab.com>
>Subject: [Histonet] RE: Balance and Scale Calibration
>To: "Piche, Jessica" <jpiche <@t> wtbyhosp.org>,
>         "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> 
><14E2C6176416974295479C64A11CB9AE019C79ECD156 <@t> SBS2K8.premierlab.local>
>Content-Type: text/plain; charset="us-ascii"
>
>Jessica
>
>Your operating manual for your balance should list your level for 
>tolerance and any good calibration company will also know what is 
>acceptable, this tolerance level can also be governed by your SOP 
>too.    We have a Mettler Toledo Model AB204-S that measures out to 
>0.0000 the tolerance on our calibration report is +/- 0.0005.   We 
>are not a clinical lab but we have our balance calibrated once a 
>year and we also use traceable weight sets prior to and after each 
>time we use the balance.  The traceable weight sets are also 
>calibrated as required.  Once you order them they come with a 
>recalibration date and then its yearly after that.  If the scale or 
>balance is not within limits and the variation cannot be corrected 
>then you would need to retire the instrument and purchase a new 
>one.  If the instrument can be adjusted then the calibration report 
>would list that the instrument "as found"  did not pass calibration 
>but was adjusted and then was calibrated "as left".  For us in a GLP 
>environment if a particular piece of equipment is found to be out of 
>tolerance we need to document that and also determine if it had any 
>effect on any GLP study that that piece of equipment was used 
>on.  If you use your balance or scale a lot then you may consider a 
>twice a year calibration.  For us we calibrate the majority of our 
>instruments yearly except for our pipettors which we calibrate quarterly.
>
>I hope this helps
>
>Liz
>
>Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
>Premier Laboratory, LLC
>PO Box 18592
>Boulder, CO 80308
>(303) 682-3949 office
>(303) 682-9060 fax
>(303) 881-0763 cell
>liz <@t> premierlab.com
>www.premierlab.com
>
>March 10, 2014 is Histotechnology Professionals Day
>
>Ship to Address:
>
>Premier Laboratory, LLC
>1567 Skyway Drive, Unit E
>Longmont, CO 80504
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
>Sent: Wednesday, June 18, 2014 10:37 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Balance and Scale Calibration
>
>Hi Everyone,
>
>I was wondering what other anatomic pathology labs are doing to 
>calibrate their scales and balances? I also would like to know what 
>the basic tolerances for balances or scales is? I can only seem to 
>find information in laboratories that deal with soil. I have a 
>procedure and a calibration record sheet in place but I'm not sure 
>what the range is for basic tolerances of the scale or balance so 
>I'm not sure what to do if a scale or balance is not within the right limits.
>
>Thank you for any insight, procedures, information etc.
>
>Jessica Piche, HT(ASCP)
>Waterbury Hospital
>
>
>
>
>CONFIDENTIALITY NOTICE: This email and any attachments contain 
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>information in error, please notify the sender immediately and 
>delete these documents. Copyright (c) Waterbury Hospital 
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>------------------------------
>
>Message: 2
>Date: Wed, 18 Jun 2014 17:54:37 +0000
>From: Matthew Lauterbach <Matthew.Lauterbach <@t> avera.org>
>Subject: [Histonet] Full Time Day Shift HT/HTL Position in Sioux
>         Falls,  South Dakota
>To: "'Histonet <@t> lists.utsouthwestern.edu'"
>         <Histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>         <0FFD9B96579AA64D9A452478AFB414E2020A751B <@t> AHDC374ML4.averamail.net>
>Content-Type: text/plain; charset="us-ascii"
>
>http://www.avera.org/mckennan/jobs/
>
>Histology Technician -Req Num: 1402696
>Category:
>         Technical/Professional (Clinical)
>Facility:
>         Avera McKennan Hospital & University Health Center
>Department:
>         Lab Histology
>Schedule:
>         Full Time
>Shift:
>         Day Shift
>Hours:
>         5:00am-1:30pm, 5:30am-2:00pm, 7:30am-4:00pm, 8:30am-5:00pm
>Requisition:
>         1402696
>Job Details:
>         SUMMARY: The Histology Technician performs standardized 
> histotechnology procedures under supervision by the Histology 
> Section Supervisor and Section Pathologist.
>
>EDUCATION and/or EXPERIENCE:
>         * An Associate's degree in Histology is preferred.
>
>CERTIFICATION, LICENSURE, and/or REGISTRATIONS:
>         * Certification as a Histology Technician (HT) from the 
> American Society Clinical Pathology (ASCP) certification is 
> required or must be received within one year of     employment or 
> the completion of a National Accrediting Agency for Clinical 
> Laboratory Sciences (NAACLS) accredited Histotechnician program, 
> whichever occurs last.
>
>
>Matthew J. Lauterbach, MBA, MLS (ASCP)CM
>Laboratory Services Manager
>Phlebotomy/Campus Services/Histology/POC
>Avera McKennan Region
>Phone: (605) 322-7126
>Cell: (605) 496-1272
>Fax: (605) 322-7083
>
>
>
>
>------------------------------
>
>Message: 3
>Date: Wed, 18 Jun 2014 19:20:30 +0000
>From: "Mayer,Toysha N" <TNMayer <@t> mdanderson.org>
>Subject: [Histonet] RE: control slides
>To: "'histonet <@t> lists.utsouthwestern.edu'"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> 
><47E9B2C01DDDD94881EACD2DC44EBC881B3B4854 <@t> D1PWPEXMBX05.mdanderson.edu>
>Content-Type: text/plain; charset="us-ascii"
>
>Juan,
>
>If you don't need them soon, I might be able to help you.  We are 
>going to be clearing out some of our slides and taking pictures of them.
>After I check with my PD I will see if we can send you some.
>You can contact me offline at: tnmayer <@t> mdanderson.org
>I will get back with you next week sometime and let you know.
>
>Sincerely,
>
>Toysha N. Mayer, MBA, HT (ASCP)
>Instructor/Education Coordinator
>Program in Histotechnology
>School of Health Professions
>UT M.D. Anderson Cancer Center
>713.563-3481
>
>This electronic mail and any attached documents are intended solely 
>for the named addressee(s) and contain confidential information. If 
>you are not an addressee, or responsible for delivering this email 
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>
>
>------------------------------
>
>Message: 4
>Date: Wed, 18 Jun 2014 16:24:17 +0000
>From: "Bassett, Juan L CTR USARMY MEDCOM USAMRMC (US)"
>         <juan.l.bassett.ctr <@t> mail.mil>
>Subject: [Histonet] control slides
>To: "Histonet <@t> lists.utsouthwestern.edu"
>         <Histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> 
><5BEFD2C58946394F8E2A6EC3E1F86A9B15A31644 <@t> umechpanz.easf.csd.disa.mil>
>Content-Type: text/plain; charset="us-ascii"
>
>I am in need of control slides (already stained) of different type 
>special stains for teaching purposes. H & E slides also needed. If 
>you are in the Maryland area I may be able to pick up from your 
>area. If out of state I'll pick up shipping .
>
>Juan l. Bassett HT
>Histotechnician Anatomical Div.
>National Museum of Health and Medicine
>2500 Linden Lane
>Silver Spring, MD 20910
>Office 301-319-3359
>Lab      301-319-3328
>Mobile 240-505-7247
>
>Juan.l.bassett.ctr <@t> mail.mil
>
>
>
>
>
>
>
>CONFIDENTIALITY NOTICE: This email and any attachments contain 
>confidential information that is legally privileged. This 
>information is intended only for the use of the individual or entity 
>named above. The authorized recipient of this information is 
>prohibited from disclosing this information to any other party 
>unless required to do so by law or regulation. If you are not the 
>intended recipient, you are hereby notified that any disclosure, 
>copying, distribution or action taken in reliance on the contents of 
>these documents is strictly prohibited. If you have received this 
>information in error, please notify the sender immediately and 
>delete these documents. Copyright (c) Waterbury Hospital
>
>
>------------------------------
>
>_______________________________________________
>Histonet mailing list
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>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>End of Histonet Digest, Vol 127, Issue 24
>*****************************************
>
>
>
>------------------------------
>
>Message: 4
>Date: Wed, 18 Jun 2014 23:02:55 -0400
>From: Amanda Coscetti <acoscetti <@t> gmail.com>
>Subject: [Histonet] Liver bxs
>To: "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <29095B14-B76E-43C2-842E-A0C83324D11D <@t> gmail.com>
>Content-Type: text/plain;       charset=us-ascii
>
>
> >
> > Hi HistoWorld!
> > Wondering if anyone has seen/heard of this as it has happened to 
> us a couple of times in the past year and just happened again today!
> > We had a liver bx come from Radiology and we processed it as 
> usual but under the microscope our pathologist said it was 
> unreadable, the edges had artifact, and it had inconsistent 
> staining (on the H&E, iron, and trichrome  slides).  The 
> radiologist claims he put the specimen directly into formalin. All 
> of our other bxs and tissue were fine.  This was the case before 
> when it happened and we have only had this problem with liver bxs!
> >  Any ideas why this may occur?
> >
> > Thanks y'all!
> > Amanda
> > Florence SC
>
>
>
>
>
> >
>
>
>
>------------------------------
>
>Message: 5
>Date: Thu, 19 Jun 2014 14:10:21 +1000
>From: Tony Reilly <Tony.Reilly <@t> health.qld.gov.au>
>Subject: [Histonet] RE: VIP 1000
>To: "Abbott, Tanya" <TanyaAbbott <@t> catholichealth.net>,
>         "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
> 
><E8088C46632C1C489B85680DD5C0D76B028140B1BC64 <@t> EXCCMSP10.qh.health.qld.gov.au>
>
>Content-Type: text/plain; charset="iso-8859-1"
>
>Hi Tanya
>
>A number of years ago I had a fume hood which had very expensive 
>disposable filters.  I approached the company that serviced and 
>calibrated our fume extraction equipment and they were able to 
>manufacture permanent filter cartridges to suit our needs.  From 
>then on at a much lower price we purchased activated carbon to 
>replace and refill the filters as required.  Not quite the same 
>problem but could be an alternative fix.
>
>Regards
>Tony
>
>
>Tony Reilly B.App.Sc,  M.Sc
>Chief Scientist
>Anatomical Pathology
>Pathology Queensland PAH
>_________________________________________________
>Health Services Support Agency| Department of Health
>
>Building 15, Level 1,
>199 Ipswich Road
>WOOLLOONGABBA  Queensland 4102
>Ph: 07 3176 2412
>Mob: 0402139411
>Fax: 07 3176 2930
>Email: tony.reillyi <@t> health.qld.gov.au | www.health.qld.gov.au
>
>
>
>
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Abbott, Tanya
>Sent: Thursday, 19 June 2014 1:57 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] VIP 1000
>
>To go along with my last question, does anyone know where I could 
>get charcoal filters for the VIP 1000 tissue processor? I have a 
>letter from 2002 from Sakura saying parts for this will probably 
>only be available for 2 more years, so that's only 10 years 
>ago,..........!! Assuming if I can't get filters, I can't run it anyway?!
>
>Tanya G. Abbott RT (CSMLS)
>Manager Technologist, Histology/Cytology St. Joseph Medical Center 
>Reading, PA 19603-0316 ph  610-378-2635 fax 610-898-5871
>email: tanyaabbott <@t> catholichealth.net
>
>This electronic mail and any attached documents are intended solely 
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>to the sender and delete the message completely from your computer system.
>_______________________________________________
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>
>------------------------------
>
>Message: 6
>Date: Thu, 19 Jun 2014 13:03:01 +0000
>From: Mike Pence <mpence <@t> grhs.net>
>Subject: RE: [Histonet] Liver bxs
>To: 'Amanda Coscetti' <acoscetti <@t> gmail.com>,
>         "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>         <D6DF96C438FB8D42B04B2345A9452CF5FEB896CE <@t> MAIL-MB01.grhs.net>
>Content-Type: text/plain; charset="us-ascii"
>
>Ask to be present at the next liver bx time and see for yourself 
>their handling of the specimen. Sounds like it set out for some time.
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu 
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti
>Sent: Wednesday, June 18, 2014 10:03 PM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] Liver bxs
>
>
> >
> > Hi HistoWorld!
> > Wondering if anyone has seen/heard of this as it has happened to 
> us a couple of times in the past year and just happened again today!
> > We had a liver bx come from Radiology and we processed it as 
> usual but under the microscope our pathologist said it was 
> unreadable, the edges had artifact, and it had inconsistent 
> staining (on the H&E, iron, and trichrome  slides).  The 
> radiologist claims he put the specimen directly into formalin. All 
> of our other bxs and tissue were fine.  This was the case before 
> when it happened and we have only had this problem with liver bxs!
> >  Any ideas why this may occur?
> >
> > Thanks y'all!
> > Amanda
> > Florence SC
>
>
>
>
>
> >
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>------------------------------
>
>Message: 7
>Date: Thu, 19 Jun 2014 13:22:16 +0000
>From: "Breckenridge, Richard A" <Richard.Breckenridge <@t> uth.tmc.edu>
>Subject: [Histonet] Anti-PLA2r
>To: "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>         <226B9BF4FE89CB4D96DEC62F59DF97F1030B79 <@t> UTHMAIL1.uthouston.edu>
>Content-Type: text/plain; charset="us-ascii"
>
>Hello all,
>We are revisiting the Anti-PLA2r fluorescence staining for renal 
>biopsies. I am in need of a working protocol. I need to know of a 
>good antibody to use. We have unsuccessfully tried using a couple of 
>antibodies now and it is getting expensive. Any help would be 
>appreciated. Thanks.
>
>Best regards,
>
>Richard A. Breckenridge, HT(ASCP)
>Special Tissues Laboratory Manager
>University of Texas Health Science Center at Houston
>Medical School Building 2.002
>ph. 713-500-6792  fax 713-500-0733
>richard.breckenridge <@t> uth.tmc.edu<mailto:richard.breckenridge <@t> uth.tmc.edu>
>
>
>
>------------------------------
>
>Message: 8
>Date: Thu, 19 Jun 2014 09:01:36 -0600
>From: "gayle callis" <gayle.callis <@t> bresnan.net>
>Subject: [Histonet] Re:  VIP 1000 charcoal filters
>To: "'Histonet '" <Histonet <@t> lists.utsouthwestern.edu>
>Message-ID: <000001cf8bcf$5fae66f0$1f0b34d0$@bresnan.net>
>Content-Type: text/plain;       charset="us-ascii"
>
>Years ago, we went to a pet store (Petco, Petsmart) or pet section in
>Walmart, etc. for bags of bulk charcoal used in fish tank filters. I am sure
>Amazon will have this too.   The charcoal pieces were very similar to what
>was in prefilled VIP filters.   We emptied the  VIP charcoal containers
>under a hood to avoid xylene fumes captured by charcoal then refilled with
>the fish tank filter charcoal.  If you try this, note the level of charcoal
>in the boxes for quantity needed.   It was actually cheaper than buying
>prefilled boxes from the vendor's, and no shipping costs either.
>Unfortunately, Sakura discontinued charcoal "milk carton"  packaging,
>probably to avoid the fume problem during filter change - a safety factor.
>
>This is somewhat of a stop gap measure but certainly worked for us.  The
>more recent charcoal boxes are cardboard and sealed, if I remember
>correctly, but careful opening and then resealing with duct tape might work.
>However, first VIP charcoal filter containers were made of the same plastic
>as the solvent containers and not easily damaged during a change.
>
>
>
>Good luck
>
>
>
>Gayle Callis
>
>HTL/HT/MT(ASCP)
>
>
>
>
>
>
>
>
>
>------------------------------
>
>Message: 9
>Date: Thu, 19 Jun 2014 12:20:49 -0400
>From: Rena Fail <renafail2 <@t> gmail.com>
>Subject: Re: [Histonet] Liver bxs
>To: Mike Pence <mpence <@t> grhs.net>
>Cc: "histonet <@t> lists.utsouthwestern.edu"
>         <histonet <@t> lists.utsouthwestern.edu>
>Message-ID:
>         <CAE7Zv_pZc5NrC=fxxHCqewOwe1eLwLUeDyJ8h33ze1jSci0L5Q <@t> mail.gmail.com>
>Content-Type: text/plain; charset=UTF-8
>
>  Your description is consistent with the tissue having dried out. So
>observing their procedure may be helpful. Is it placed on gauze then placed
>in formalin ? That would dry it quickly. are they tending to the pt then
>placing the specimen in fprmalin,
>Rena Fail
>
>
>On Thu, Jun 19, 2014 at 9:03 AM, Mike Pence <mpence <@t> grhs.net> wrote:
>
> > Ask to be present at the next liver bx time and see for yourself their
> > handling of the specimen. Sounds like it set out for some time.
> >
> > -----Original Message-----
> > From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> > histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amanda Coscetti
> > Sent: Wednesday, June 18, 2014 10:03 PM
> > To: histonet <@t> lists.utsouthwestern.edu
> > Subject: [Histonet] Liver bxs
> >
> >
> > >
> > > Hi HistoWorld!
> > > Wondering if anyone has seen/heard of this as it has happened to us a
> > couple of times in the past year and just happened again today!
> > > We had a liver bx come from Radiology and we processed it as usual but
> > under the microscope our pathologist said it was unreadable, the edges had
> > artifact, and it had inconsistent staining (on the H&E, iron, and trichrome
> >  slides).  The radiologist claims he put the specimen directly into
> > formalin. All of our other bxs and tissue were fine.  This was the case
> > before when it happened and we have only had this problem with liver bxs!
> > >  Any ideas why this may occur?
> > >
> > > Thanks y'all!
> > > Amanda
> > > Florence SC
> >
> >
> >
> >
> >
> > >
> >
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> >
> >
> >
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> >
>
>
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>End of Histonet Digest, Vol 127, Issue 25
>*****************************************




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