[Histonet] rolling sections

[Lee & Peggy Wenk]

Try changing the angle of the knife blade, so that the clearance angle 
(angle between the knife blade and the block face) is larger. In other 
words, tip the top of the blade towards the block more. If there are numbers 
on the side of the knife holder, you want to move it to a larger number 
(like from 5 to 10). Before sectioning, remember to move the block holder 
towards you, since the blade will now be closer to the block, and you don't 
want to ker-chunk the block.

And remember, when done, to return the clearance angle back to it's usual 
location, so you aren't curling all your ribbons. So look at the number it 
is usually set at, or, if there is no number, make some marks on the side of 
the knife holder, that you can line up again.

Peggy A. Wenk, HTL(ASCP)SLS

-----Original Message----- 
From: Roberta Horner
Sent: Thursday, June 12, 2014 10:42 AM
To: Histonet (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] rolling sections

I have some researchers that want to do PCR.  They want 10 - 10u sections in 
a micro-centrifuge tube.  The only way to get the sections in the tube is 
for the sections to roll.  How do you get sections to roll when you want 
them to roll?  I've tried room temperature, on ice, brand new sharp blade, 
dull blade and I can still get some really nice ribbons.  When I want a 
thick ribbon it will roll, darn that Murphy and his laws.
Roberta Horner
Animal Diagnostic Lab
Penn State University
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet