[Histonet] RE: rolling sections

[Truscott, Tom]

Hi Roberta, Check with those requesting to see if you can cut the equilivant but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are lots easier to get into the tubes. Tom T 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Pam Marcum
Sent: Thursday, June 12, 2014 8:00 AM
To: Helen Fedor
Cc: Histonet (histonet <@t> lists.utsouthwestern.edu); Roberta Horner
Subject: Re: [Histonet] RE: rolling sections



That is how we do it also.  Since they are not really interested in the sections being flat we just pick them and put them directly into the tube.If I am really having issues I will actually warm the block with my finger to help it roll.  If they are doing DNA or RNAase ou may need to wear glove to prevent any contamination of the blocks.  



Pam Marcum 

UAMS 
----- Original Message -----
From: "Helen Fedor" <hfedor <@t> jhmi.edu> 
To: "Roberta Horner" <rjr6 <@t> psu.edu>, "Histonet (histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Thursday, June 12, 2014 9:54:45 AM 
Subject: [Histonet] RE: rolling sections 

Hi, I think that it is not necessary to actually get them to roll. We just collect all of the sections and put them into the tube. Scrunched, not rolled. 

Helen 

-----Original Message----- 
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Roberta Horner 
Sent: Thursday, June 12, 2014 10:42 AM 
To: Histonet (histonet <@t> lists.utsouthwestern.edu) 
Subject: [Histonet] rolling sections 

I have some researchers that want to do PCR.  They want 10 - 10u sections in a micro-centrifuge tube.  The only way to get the sections in the tube is for the sections to roll.  How do you get sections to roll when you want them to roll?  I've tried room temperature, on ice, brand new sharp blade, dull blade and I can still get some really nice ribbons.  When I want a thick ribbon it will roll, darn that Murphy and his laws. 
Roberta Horner 
Animal Diagnostic Lab 
Penn State University 
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