[Histonet] Processor problem

[Denice Stiner]

I do not have a Tissue Tek 3000 but it sounds like maybe there is a sensor that needs wiped clean.  Clearly it's not ready the fluid level in station 3 and returning it back to 1 for safe keeping.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, June 06, 2014 1:56 PM
To: Mike Tighe; histonet <@t> lists.utsouthwestern.edu (histonet <@t> lists.utsouthwestern.edu)
Subject: Re: [Histonet] Processor problem

My advise is to call Sakura and schedule a maintenance visit. The rotary valve may be "acting up".
René J. 


On Friday, June 6, 2014 1:53 PM, Mike Tighe <mtighe <@t> trudeauinstitute.org> wrote:
  


I am having a problem with our tissue processor (Tissue Tek 3000). During processing the first reagent (NBF) gets pumped into the retort without any problems and the same for the 2nd reagent (70% ETOH). The Third reagent (also 70%) however, gets about half way through pumping into retort and stops. After that it reverses and pumps out reagent three, then pumps in half of reagent 2. Pumps out the half of reagent 2 and pumps in half of reagent 1. This is followed by a low fluid warning.



I have removed and reinserted the reagent reservoirs and suction tube. The tubing is not caught in the handle and seems to be fine. I have created a short (5min) automatic run with 5 minutes per station (no samples) and this problem repeats itself.



Has anybody had this problem?



Thanks for any help!

Mike
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