[Histonet] Validating slides

[Michael Ann Jones]

P.S. We use Platinum series from Mercedes Medical (so far we're okay)
previously we used Fisher Superfrost +, good quality, however these were
very expensive.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones <@t> metropath.com




On 6/4/14 4:48 PM, "Sharon Scalise" <SSCALISE <@t> beaumont.edu> wrote:

>I am looking for comments about validating slides in histology/immuno.
>We have always validated our charged slides if we went to a new
>brand/vendor in order to ensure that the tissue sections will adhere
>properly during immuno procedures and certain special stains.  Once we
>found them to be acceptable, we continue to use them, lot to lot, and
>never validate as long as we do not change brand/vendor.  We recently ran
>into a problem with slides that we received by accident (similar
>packaging, different slide) and they were used for some immuno cases.
>The sections on many of the slides fell off and the stains had to be
>repeated.  We realized the problem and pulled the slides out of service.
>We contacted the vendor and they said that those slides should have been
>pulled from the shelf, that it was a bad batch and they should have never
>been sent to anyone, yet alone incorrectly sent to us.
>I started to think that we might want to consider validating new lots we
>receive to ensure that each lot is acceptable.  As we began this
>discussion there were people who felt that in any lot you could have
>slides that are acceptable and maybe some boxes that give you problems.
>How do you know if all slides in one lot are treated exactly the same?
>Can you really use lot number to verify quality when it comes to slides?
>I am not exactly sure of the process used to "charge" the slides so I
>don't feel qualified to answer these questions.
>What is everyone else doing about slide validation?  Is it really
>necessary? (I can say that in 30 years I have never seen it done with
>each new lot, but I have been at the same job for the past 18 years).
>
>Thanks for your input!
>
>Sharon Scalise, HTL(ASCP)
>Histology Supervisor-Anatomic Pathology
>Beaumont Health System
>3601 W. 13 Mile Rd.
>Royal Oak, MI 48073
>248 898-5981
>sscalise <@t> beaumont.edu<mailto:sscalise <@t> beaumont.edu>
>
>
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