[Histonet] Schiff : room temp or not

[Hans B Snyder]

Hello Amber,

That depends on your protocol. Which protocol are you using and what
does your protocol say to use the schiffs at?  Consider that schiffs
is usually stored at 4C in brown or opaque bottles.  This suggests it
is light sensitive.  Our protocol say to use the schiffs at 37C for 15
minutes but I have used it at 22C for 30 minutes and at 60C for 10
minutes with consistent results.  We have found that using it at 60C
reduces it's shelf life considerably and do not suggest it unless you
are familiar with doing so.

Good luck
Hans B Snyder
Histologistics
60 Prescott Street
Worcester, MA 01605
508-308-7800
hans <@t> histologistics.com


On Wed, Jul 30, 2014 at 4:13 PM, Amber McKenzie
<amber.mckenzie <@t> gastrodocs.net> wrote:
>
> When staining PAS's, does the Schiff need to be room temp or does it matter?  Just curious about when to add it to our Sakura stainer.  Should we put the schiff's on at the beginning of our shift so it's room temp when we need it or when should add it when we add PAS slides to it that need to be stained? Thanks!
>
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