[Histonet] Mouse on Mouse Staining

[John Shelley]

Good Morning,

I have avoided for the last few years doing IHC with mouse on mouse antibodies, but have been requested to do so at this time. I have two antibodies, HDAC1 and Nitrotyrosine from Santa Cruz that I am working up, however the staining pattern in my tissue does not seem to be revealing the accurate staining pattern. I was hoping someone in the research community has worked with these antibodies, even if from other vendors and would be willing to discuss further with me. Thanks!!!

Kind Regards!

John J Shelley
Research Specialist, Histology Core Facility
Sanford-Burnham Medical Research Institute at Lake Nona