[Histonet] HS IHC project

[Emily Brown]

I am so jealous of these kids.  I didn't get near a microscope until
college, let alone a microtome.  I hope they have fun!

Emily

"By bitching and bitching and bitching, they could exhaust the drama of
their own horror stories. Grow bored. Only then could they accept a new
story for their lives. Move forward."

-Chuck Palahniuk, "Haunted"


On Mon, Jul 21, 2014 at 2:19 PM, Jennifer MacDonald <JMacDonald <@t> mtsac.edu>
wrote:

> T cell and B cell markers work well with human spleen.
>
>
>
> From:   Patsy Ruegg <pruegg <@t> ihctech.net>
> To:     "Histonet <@t> Lists. Edu" <histonet <@t> lists.utsouthwestern.edu>
> Date:   07/21/2014 11:08 AM
> Subject:        [Histonet] HS IHC project
> Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu
>
>
>
> Friends,
>
> I am going to help a local HS class (a Biotech class) do
>  a simple IHC project.  This class already processes tissue, embeds,
> sections and does an H&E stain.  My husband fixed up an old black AO
>  microtome for them to use and they actually cut sections.  They cannot
> use human tissue there so what they have available is mouse spleen
> already ffpe.  I was thinking of using a rab antibody that works on ffpe
>  ms spleen.  Ki67 comes to mind?  It has been a while since I was in the
>  lab so I am consulting with you all to make sure a rab poly or rab mono
>  Ki67 will indeed stain a ffpe ms spleen?  If you have evidence of this
> can you tell me which ab was used successfully.  If you can think of a
> better rab ab to use on the ffpe ms spleen for this project let me
> know.  It would help if we could keep it simple for antigen retrieval.
>
> One of our kind vendors will be donating IHC reagents for this HS class
> project.
>
> I hope the IHC meeting is going well in Vegas, wish I was there.
>
> Cheers,
> Patsy
>
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting
> 40864 E Arkansas Ave
> Bennett, CO 80102
> H 303-644-4538
> C 720-281-5406
> pruegghm <@t> hotmail.com
> pruegg <@t> ihctech.net
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