[Histonet] RE: Storage of HER2 neu control slides

[Joelle Weaver]

I do the complete drain & dry, date, then refrigeration. Use them within 6 weeks. The Oracle IVD, IFU says bake overnight, but I don't see that happening too often. 


Joelle Weaver MAOM, HTL (ASCP) QIHC

        
  

 
> From: Richard.Cartun <@t> hhchealth.org
> To: LSebree <@t> uwhealth.org; Histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 14 Jul 2014 14:22:43 +0000
> CC: 
> Subject: [Histonet] RE: Storage of HER2 neu control slides
> 
> We keep all of our positive control slides at RT.  The breast predictive marker slides often sit for a few weeks (after cutting) before being used.  I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides.
> 
> Richard
> 
> Richard W. Cartun, MS, PhD
> Director, Histology & Immunopathology
> Director, Biospecimen Collection Programs
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT  06102
> (860) 972-1596 Office
> (860) 545-2204 Fax
> richard.cartun <@t> hhchealth.org
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
> Sent: Monday, July 14, 2014 8:44 AM
> To: Histonet (Histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] Storage of HER2 neu control slides
> 
> Good morning,
> 
> I'd like to get some opinions on the storage of positive control slides for HER2 neu.  We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind.  What has peoples' experiences been with the storage of these control slides?
> 
> Thanks for any and all replies,
> 
> Linda
> 
> 
> Linda A. Sebree
> University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
> 
> 
> 
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