[Histonet] RE: Storage of HER2 neu control slides

Cartun, Richard Richard.Cartun <@t> hhchealth.org
Mon Jul 14 09:22:43 CDT 2014

We keep all of our positive control slides at RT.  The breast predictive marker slides often sit for a few weeks (after cutting) before being used.  I think everyone's situation will be different due to laboratory humidity, tissue fixation, and tissue processing which removes the water from the tissue which has been shown to be the one of the culprits in antigen degradation in stored unstained slides.


Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596 Office
(860) 545-2204 Fax
richard.cartun <@t> hhchealth.org

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sebree Linda A
Sent: Monday, July 14, 2014 8:44 AM
To: Histonet (Histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] Storage of HER2 neu control slides

Good morning,

I'd like to get some opinions on the storage of positive control slides for HER2 neu.  We currently keep a number of positive control slides in a -20 degree freezer as some antigens tend to lose reactivity at room temperature; ER and PR come to mind.  What has peoples' experiences been with the storage of these control slides?

Thanks for any and all replies,


Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792
FAX: (608)262-7174

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