[Histonet] Wright-Geimsa Stain
Rene J Buesa
rjbuesa <@t> yahoo.com
Sat Jul 12 09:16:24 CDT 2014
Are you using smears or tissue sections? Imagining you are using the former; eosin color in RBC should vary according with:
1- "age" of the RBC (after losing their nucleus);
2- staining technique (time, fixation and "age" of the solution);
3- type and concentration of eosin.
So I think that you first should create a standard technique (including the one to measure "color intensity") and latter try your idea.
For me it is very interesting why do you want to do this?
On Friday, July 11, 2014 3:26 PM, jerry sedgewick <jerrysedgewick <@t> gmail.com> wrote:
I'm doing a project where I'm measuring color differences from histology
session to histology session. Not being a histologist, I'm not certain
whether RBCs can be used as a feature to measure for color change with
Wright-Giemsa. I suppose it depends on the Eosin Y concentration, but,
even at low concentrations, should I see RBCs stained?
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