[Histonet] Wright-Geimsa Stain

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Jul 12 09:16:24 CDT 2014

Are you using smears or tissue sections? Imagining you are using the former; eosin color in RBC should vary according with:
1- "age" of the RBC (after losing their nucleus);
2- staining technique (time, fixation and "age" of the solution);
3- type and concentration of eosin.
So I think that you first should create a standard technique (including the one to measure "color intensity") and latter try your idea.
For me it is very interesting why do you want to do this?
René J. 

On Friday, July 11, 2014 3:26 PM, jerry sedgewick <jerrysedgewick <@t> gmail.com> wrote:

I'm doing a project where I'm measuring color differences from histology 
session to histology session.  Not being a histologist, I'm not certain 
whether RBCs can be used as a feature to measure for color change with 
Wright-Giemsa.  I suppose it depends on the Eosin Y concentration, but, 
even at low concentrations, should I see RBCs stained?


Jerry Sedgewick
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