[Histonet] marking tiny specimens

[Truscott, Tom]

Hi Cheryl, If you can, use more eggs. Then process them into a pellet with histogel. Upon sectioning, many different cross-sections will be visible in the same section. Tom T

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Thursday, January 30, 2014 12:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are eggs with a protein covering.  I have tried using eosin to color the tissues before processing but the color came out before paraffin.  The coating on the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed.  Thanks in advance, Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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