[Histonet] marking tiny specimens

[Elizabeth Chlipala]

Cheryl

So the eggs don't absorb the eosin at all or does the dye come out during processing?  We use eosin all of the time for samples like that, but I leave them in eosin for about 10 minutes.  Here is what we do, the fixed samples are normally submitted in Eppendorf tubes, we remove the formalin and replace with eosin (I use Anatechs), let sit for about 10 minutes and then very gently we use a plastic disposable pipette (the ones with bulbs) we place the samples in a tea bag and process on a very short cycle on the tissue processor (5 -10 minutes per station) we do this during the day so it's a continuous process.

Liz  

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz <@t> premierlab.com
www.premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cheryl Crowder
Sent: Thursday, January 30, 2014 1:00 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] marking tiny specimens

I am processing some extremely small specimens - pin tip size.  These are eggs with a protein covering.  I have tried using eosin to color the tissues before processing but the color came out before paraffin.  The coating on the eggs will not absorb the dye.  Does anyone have a suggestion for dyeing or marking these tissues so I can see them better to embed.  Thanks in advance, Cheryl

Cheryl Crowder, BA, HTL(ASCP)

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