[Histonet] Wright Stain on paraffin sections

[John Kiernan]

For formaldehyde-fixed material the pH has to be lower than for methanol-fixed blood or marrow smears (for which pH6.8 is best).  
For your sections try pH 5.0 first. Go down in steps of 0.5 if too blue; up in steps of 0.5 if too red. The colour scheme is not a varied as with a blood film, but it's prettier by far than H&E. 
 
John Kiernan
Anatomy, UWO
London, Canada
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On 23/01/14, "Berger, Rebecca" <BergerR1 <@t> email.chop.edu> wrote: 
>  Hi all,
> I am trying to look at eosinophils and neutrophils in FFPE muscle sections. Does anyone have experience with the Wright stain or a Wright staining kit on these types of sections? Or possibly a suggestion for a different protocol to visualize these cells?
> Thanks!
> Becky
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