[Histonet] How to process tissues submitted in acrylamide
John Kiernan
jkiernan <@t> uwo.ca
Fri Jan 24 14:02:32 CST 2014
If it's a cross-linked polyacrylamide gel (as used, for example, for electrophoresis), the block will suddenly shrink to a tiny stone-like object when the water content reaches a critical low level (15-20% water, 80-85% alcohol). I may have the % wrong; remembering an experiment in the early 1980s. You will need to do frozen sections. For technical details see Hausen,P & Dreyer,C (1981) Stain Technol. 56: 287-293. They didn't cryoprotect. Evidently its important not to let the sections dry out on the slides (or coverslips) before staining.
John Kiernan
Anatomy, UWO
London, Canada
= = =
On 23/01/14, Catherine Simonson <catherinesimonson <@t> gmail.com> wrote:
> Hello Helpful Histonetters,
>
> I have some samples that were fixed in PFA, placed in acrylamide gel and
> then treated with iodine for microCT at another facility. The investigator
> now wants to follow up and get H&E's on these samples. I know how to deal
> with the iodine, but my question is this: what is the best procedure to
> process these samples to slide? Do I run on a long processing schedule on
> the processor, or do I do sucrose cryoprotection and get frozen.
> Morphometry seems to be an important factor.
>
> Thanks in advance,
>
> Catherine Simonson, B.S., HT(ASCP)
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