[Histonet] Silver in Nucleolus

[Tony Henwood (SCHN)]

Hi Jerry,

Are you sure they are not AgNOR's:

Lindner, L. E. (1993). Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). Journal of Histochemistry & Cytochemistry, 41(3), 439-445.

Thiebaut, F., Rigaut, J. P., & Reith, A. (1984). Improvement in the Specificity of the Silver Staining Technique for Ag NOR-ASSOCIATED Acidic Proteins in Paraffin Sections. Biotechnic & Histochemistry, 59(3), 181-185.

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 

Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of jerry sedgewick
Sent: Friday, 24 January 2014 8:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Silver in Nucleolus

I'm working with a lab that stained cat gonads with Warthin Starry. 
We're looking for Bartonella bacteria.  In the first batch that was stained, some nucleii filled with the silver stain, something I've see in published images.  In the second batch it appears that nucleolus filled with silver.  I haven't seen that in google searches, etc.  I wondered if others have seen the nucleolus fill with silver (or is this bartonella?).  I'm also wondering how to prevent this sort of thing with proper bench procedures.

Thanks!

Jerry Sedgewick


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