[Histonet] IHC-start-up

[Jan Shivers]

I work in animal tissue only.  I also agree that an open autostainer system
works best, since you may have to run multiple dilutions and incubation
times for the same antibody because of the different species' reactivities.
 You may also need multiple types of reagents to cover detection in
different species, so you'll need a system which will allow you to use
multiple vendor products.

An aside... The CD4 and CD8 Abs that I've done on various animals are
usually only done on frozen sections, so I tend to perform that IHC
manually, in order to be able to rinse slides more gently than an
autostainer does (and do overnight incubation with primary Ab, if
necessary).

Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu


On Thu, Jan 23, 2014 at 12:12 PM, James Watson <JWatson <@t> gnf.org> wrote:

> Erin,
>
> We run the Ventana Discovery XT at our lab and average 15,000 antibodies
> stained a year using 5 stainers on animal tissue.  If you are working on
> volume I would also look at the Leica Bond system.   Leica and Ventana
>  both have released or are releasing more flexible systems as far as
> programing staining protocols.  With the Ventanas you are tied into their
> chromogen labeling systems and LCS,  but most other reagents can be
> substituted.
>
> Other systems are more open,  but do not offer on line deparaffinization
> or HEIR.  These would be great if not working with large volume.
>
> The Ventanas have worked great for us, but like all stainers they do have
> their little idiosyncrasies.
>
> Look at what fits your needs and look closely at the cost of running a
> system vs. efficiency.
>
> Jamie
>
> James Watson HT  ASCP
> GNF  Genomics Institute of the Novartis Research Foundation
> Tel    858-332-4647
> Fax   858-812-1915
> jwatson <@t> gnf.org
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Will Chappell
> Sent: Thursday, January 23, 2014 9:45 AM
> To: Erin Sarricks
> Cc: histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] IHC-start-up
>
> Doing ihc on animal tissue is very tricky, if for no other reason than you
> are trying to standardize an antibody across multiple species. You will
> need to use every trick in the book and invent some of your own to get
> reproducible results.
>
> I would steer clear of any platforms that do not let you customize
> everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have
> their quirks but they have the openness you require.
>
> William Chappell
>
> Sent from my iPhone
>
> > On Jan 23, 2014, at 11:49 AM, Erin Sarricks <esarricks <@t> gmail.com> wrote:
> >
> > Hi all-
> >
> > Our histopathology lab is looking to
> > set up an IHC component to service the request of our clients.  All work
> is on animal tissue.  We will probably run about 1,000 IHC slides the first
> year and hope to increase the workload each year.  Some  stains we would
> run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana
> Discovery XT and appeared to be a good fit for our needs.  Does anyone have
> any other advice on other machines we should look into?  Any information or
> advice would be greatly appreciated. Thank you!
> >
> > Regards,
> >
> > Erin Sarricks, HT (ASCP)
> > _______________________________________________
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>
>