[Histonet] IHC-start-up

[James Watson]

Erin,

We run the Ventana Discovery XT at our lab and average 15,000 antibodies stained a year using 5 stainers on animal tissue.  If you are working on volume I would also look at the Leica Bond system.   Leica and Ventana  both have released or are releasing more flexible systems as far as programing staining protocols.  With the Ventanas you are tied into their chromogen labeling systems and LCS,  but most other reagents can be substituted.

Other systems are more open,  but do not offer on line deparaffinization or HEIR.  These would be great if not working with large volume.

The Ventanas have worked great for us, but like all stainers they do have their little idiosyncrasies.  

Look at what fits your needs and look closely at the cost of running a system vs. efficiency.

Jamie

James Watson HT  ASCP
GNF  Genomics Institute of the Novartis Research Foundation
Tel    858-332-4647
Fax   858-812-1915
jwatson <@t> gnf.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Will Chappell
Sent: Thursday, January 23, 2014 9:45 AM
To: Erin Sarricks
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] IHC-start-up

Doing ihc on animal tissue is very tricky, if for no other reason than you are trying to standardize an antibody across multiple species. You will need to use every trick in the book and invent some of your own to get reproducible results. 

I would steer clear of any platforms that do not let you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. 

William Chappell 

Sent from my iPhone

> On Jan 23, 2014, at 11:49 AM, Erin Sarricks <esarricks <@t> gmail.com> wrote:
> 
> Hi all-
> 
> Our histopathology lab is looking to
> set up an IHC component to service the request of our clients.  All work is on animal tissue.  We will probably run about 1,000 IHC slides the first year and hope to increase the workload each year.  Some  stains we would run would include CD4, CD8, Ki-67 and TUNEL. We looked at the Ventana Discovery XT and appeared to be a good fit for our needs.  Does anyone have any other advice on other machines we should look into?  Any information or advice would be greatly appreciated. Thank you!
> 
> Regards,
> 
> Erin Sarricks, HT (ASCP)
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> Histonet <@t> lists.utsouthwestern.edu
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