[Histonet] Re: Which] Buffer for IHC

[Jan Shivers]

To calibrate a pH meter, we use purchased pH buffers at pH 4.0, 7.0, and
10.0.

To perform the IHC method, we use TBS (pH 7.6) with Tween 20 as our rinse
buffer.

Jan Shivers


On Thu, Jan 23, 2014 at 2:52 AM, Edwards, Richard <ree3 <@t> leicester.ac.uk>wrote:

> And what  is the buffer of choice, PBS, TBS, with or  without
> protein/detergents or whatever, thanks.
>
>
>                                                                Richard
>  Edwards
>
>
> Leicester University
>
>
>
>    U.K.
>
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
> Sent: 22 January 2014 22:08
> To: Laurie Colbert
> Cc: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
> Subject: Re: [Histonet] Buffer pH for IHC
>
> pH meter; Corning.
>
>
> On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert <lcolbert <@t> pathmdlabs.com
> >wrote:
>
> > What does everyone use to test the pH of their buffers for IHC?  Are
> > pH strips adequate or do you have to have an actual pH meter?  If you
> > use a meter, any suggestions on a make and model?
> >
> > Laurie Colbert, HT (ASCP)
> > Histology Supervisor
> > PATH MD
> > 8158 Beverly Blvd.
> > Los Angeles, CA  90048
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>
> --
> Jan Shivers
> Senior Scientist
> IHC/Histology Section Head
> Pathology Teaching Program
> Veterinary Diagnostic Laboratory
> University of Minnesota
> 1333 Gortner Ave.
> St. Paul, MN  55108
> 612-624-7297
> shive003 <@t> umn.edu
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-- 
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu