[Histonet] Which] Buffer for IHC

[Edwards, Richard]

And what  is the buffer of choice, PBS, TBS, with or  without protein/detergents or whatever, thanks.
            
                                      
                                                               Richard  Edwards

                                                                    Leicester University


                                                                             U.K.


                                                                                

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: 22 January 2014 22:08
To: Laurie Colbert
Cc: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
Subject: Re: [Histonet] Buffer pH for IHC

pH meter; Corning.


On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert <lcolbert <@t> pathmdlabs.com>wrote:

> What does everyone use to test the pH of their buffers for IHC?  Are 
> pH strips adequate or do you have to have an actual pH meter?  If you 
> use a meter, any suggestions on a make and model?
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA  90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
>
> The information contained in this transmission may contain privileged 
> and confidential information, including patient information protected 
> by federal and state privacy laws. It is intended only for the use of 
> the
> person(s) named above. If you are not the intended recipient, you are 
> hereby notified that any review, dissemination, distribution, or 
> duplication of this communication is strictly prohibited. If you are 
> not the intended recipient, please contact the sender by reply email 
> and destroy all copies of the original message.
>
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>



--
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet