[Histonet] Which] Buffer for IHC

Edwards, Richard ree3 <@t> leicester.ac.uk
Thu Jan 23 02:52:04 CST 2014


And what  is the buffer of choice, PBS, TBS, with or  without protein/detergents or whatever, thanks.
            
                                      
                                                               Richard  Edwards

                                                                    Leicester University


                                                                             U.K.


                                                                                

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: 22 January 2014 22:08
To: Laurie Colbert
Cc: Histonet Post (histonet <@t> lists.utsouthwestern.edu)
Subject: Re: [Histonet] Buffer pH for IHC

pH meter; Corning.


On Wed, Jan 22, 2014 at 3:21 PM, Laurie Colbert <lcolbert <@t> pathmdlabs.com>wrote:

> What does everyone use to test the pH of their buffers for IHC?  Are 
> pH strips adequate or do you have to have an actual pH meter?  If you 
> use a meter, any suggestions on a make and model?
>
> Laurie Colbert, HT (ASCP)
> Histology Supervisor
> PATH MD
> 8158 Beverly Blvd.
> Los Angeles, CA  90048
> (323) 648-3214 direct
> (424) 245-7284 main lab
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--
Jan Shivers
Senior Scientist
IHC/Histology Section Head
Pathology Teaching Program
Veterinary Diagnostic Laboratory
University of Minnesota
1333 Gortner Ave.
St. Paul, MN  55108
612-624-7297
shive003 <@t> umn.edu
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