[Histonet] Re: Histogel

[Teri Johnson]

Hi Jennifer,

We use low melt point agarose to pre-embed and it works consistently well with our processing.


Material:

*                     10% Neutral Buffered Formalin

*                     1X Dulbeccos PBS

*                     NuSieve GTG Low Melting Point Agarose: Lonza, Cat# 50080

Solutions:

1% Neutral Buffered Formalin

10% Neutral Buffered Formalin.......................................................10 ml

1X DPBS.......................................................................................90 ml

3.0% Agarose

NuSieve Low Melting Point Agarose powder..........................3.0 gm

1% Neutral Buffer Formalin...................................................100.0 ml

1.        In a fume hood, warm up the mixture until the agarose completely dissolves and maintain the solution at approximately 40 degrees C.

2.        Aliquot into tubes and store at room temperature.

3.        Prior to use, warm the agarose to 40 degrees C until completely melted.


Teri Johnson
Manager, Histology
Genomics Institute for
Novartis Research
Foundation
San Diego, CA
858-332-4752