[Histonet] Cryostat

[Hugh Luk]

Tanya and Madeline,

Jan Minshew of Leica sent a nicely detailed response to "Decontaminating" a cryostat in 
January of 2010. She apparently wrote it to put in a Leica "How To" manual, although I did
not notice it in this form.  Everything she states is well documented, although I also 
incorporate a known virucide and tuberculocidal product (following it's directions, naturally).

http://lists.utsouthwestern.edu/pipermail/histonet/2010-January/048750.html

If you have a UV cryostat, use Leica's Cryofect product or follow Tim Morken's previous
(2000 histosearch)formaldehyde method, they should work for killing most bugs too(with the 
exception of eradicating some prions like CJD/BSE).


If I am misunderstanding what you mean by "Decon" I apologize, as there is no reason why
you cannot clean up tissue debris in a cold cryostat with 100% alcohol.

Hugh
Hawaii

------------------------------
 
Date: Thu, 16 Jan 2014 10:43:09 -0800 (PST)
From: Madeline Gi <madelinegi <@t> yahoo.com>
Subject: Re: [Histonet] Cryostat
To: "Abbott, Tanya" <TanyaAbbott <@t> catholichealth.net>,
	"Histonet <@t> lists.utsouthwestern.edu"
	<Histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1389897789.16602.YahooMailNeo <@t> web121504.mail.ne1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1
 
I use to decon my cryostat with 100% Alco this keeps it from freezing up and it clean it. 
best of luck. 
 
Madeline Rotger Milanese H.T. BSHCS
500 New Hempstead Rd.
New City N.Y. 10965
845-362-3200 Ext 129
madelinegi <@t> yahoo.com
 
 
 
On Wednesday, January 15, 2014 2:52 PM, "Abbott, Tanya" <TanyaAbbott <@t> catholichealth.net> wrote:
 
Need to decon my cryostat
 
Tanya G. Abbott RT (CSMLS)
Manager Technologist, Histology/Cytology
St. Joseph Medical Center
Reading, PA 19603-0316
ph  610-378-2635
fax 610-898-5871
email: tanyaabbott <@t> catholichealth.net
 
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