[Histonet] Warthin Starry Help
Campbell, Tasha M.
tmcampbell <@t> fmh.org
Tue Jan 14 08:55:24 CST 2014
I need help with warthin starry stain please. I am a very small lab
trying to save money so I want to make the reagents myself. I have
sigma Aldrich dry reagents and have been making the reagents and
following the instructions correctly and I did get the stain to work
wonderfully when I first started. Then suddenly it quit working and I
cannot get it to work again. I have made fresh reagents day after day
and I have a pH of 3.8 for the acidulated water. The tissue will stain
normally with the yellow to brown background and I the developing
solution turns black when it is left on paper but the h.pylori organisms
are not staining. I am getting very frustrated and need some help
please.
Thank you.
Tasha Campbell, HTL
tmcampbell <@t> fmh.org
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