[Histonet] Soaking artifact

[Susan.Walzer <@t> HCAHealthcare.com]

Incredible! Lee Luna (who wrote the book on histology) always said" rehydrate! rehydrate, rehydrate" Where are these so called supervisors coming from??? 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of b427297 <@t> aol.com
Sent: Sunday, January 05, 2014 9:41 PM
To: dea.leslie <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Soaking artifact

Your supervisor is wrong, and inexperienced.  What artifact?  Some tissues MUST be soaked on wet ice, spleen, liver, eye lens, anything bloody -   You just can't get quality sections without soaking some tissues. You can tell her I said so.  I'd like to know what artifact she is 'seeing'.
Jackie O'Connor



-----Original Message-----
From: Deanna Leslie <dea.leslie <@t> gmail.com>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Sun, Jan 5, 2014 3:44 pm
Subject: [Histonet] Soaking artifact


Has anybody in histoland ever heard of this?  I have been cutting tissue for 25 yrs and until recently I had never heard of this!
I am under contract to a facility and the supervisor there does not want anybody to soak their tissue or use ice!  Your are supposed to use the cold plate, because as I have stated soaking them causing an artifact. I have not disputed this because it is not my place or in my job discription as a traveler.  I am not even sure what it is supposed to look like or what type of problems it causes.

Thanks for listening!
Deanna Leslie HT ASCP
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