[Histonet] Wierd hemotoxylin result on my macaque liver slide

[Lewis, Patrick]

Hi everyone.

I was staining a FFPE macaque liver tissue slide with hematoxylin the other day, and it had an odd appearance.

All the cells stained the usual way with the blue nuclear staining that you expect with hematoxylin.

But a few randomly scattered individual cells throughout the tissue stained a sharp nuclear dark black.

The cells are not clustered in any way, the cells have sharp black nuclear staining.  It's definitely not artifact.

Its not a cluster of lymphoid cells like you would see in inflammation.

What would cause seemingly random individual cells scattered throughout the tissue to stain black with just hematoxylin?

Just curious

Patrick.


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