[Histonet] waves in the surface of vibratome cuttings

[Mariela Chertoff]

Hi averyone,

We cut some mouse brains (postfixed 24hrs in PFA 4%), we cut at 40um and
when we made Nissl staining we obseved waves of staining (more and less
staining frames). We cut at 70Hz, amplitud 0.8mm and spead 0.025mm/sec.
I believe that is a problem at the moment of cutting, but we don´t know how
to solve this problem? Any advice?

Thak you very much for your help!!!


 Mariela Chertoff, PhD
Laboratorio de Biología Molecular - QB75
Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales - UBA
Ciudad Universitaria Pabellón II Piso 4
Ciudad de Buenos Aires - Argentina
Tel: 54 11 4576-3300/09 - Int. 221
email: marielachertoff <@t> qb.fcen.uba.ar
marielachertoff <@t> gmail.com
<mariela.chertoff <@t> uab.cat>